Quantificação do interferon-tau durante o reconhecimento materno da gestação em fêmeas bovinas Bos taurus indicus

Autor: Giassetti, Mariana Ianello, Pontes, Eduardo Oliveira, Niemeyer, Claudia, Siqueira, Adriano Felipe Perez, Fedozzi, Filipe, Lima, Marcelo Cardoso, Marques, Vanessa Belentani, Bertan, Claudia Maria, Miglino, Maria Angélica, Arruda, Rubens Paes, Papa, Paula Carvalho, Binelli, Mario
Jazyk: portugalština
Rok vydání: 2008
Předmět:
Zdroj: Brazilian Journal of Veterinary Research and Animal Science; Vol. 45 (2008): Suplemento; 111-120
Brazilian Journal of Veterinary Research and Animal Science; v. 45 (2008): Suplemento; 111-120
Brazilian Journal of Veterinary Research and Animal Science; V. 45 (2008): Suplemento; 111-120
Brazilian Journal of Veterinary Research and Animal Science
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron:USP
ISSN: 1678-4456
1413-9596
Popis: During the critical period of the maternal recognition, which occurs between days 15 and 19 of pregnancy, the conceptus must competently synthesize molecules capable of blocking the synthesis of prostaglandin F2α (PGF2α) and luteolysis. In cattle, the major macromolecule involved in suck blockage is the protein interferon-tau (IFN-τ). During the critical period, failures in the recognition of pregnancy determine embryonic mortality on up to 40% of inseminated cows. Data about IFN-τ in Bos taurus indicus are still scarce. Objective of this study was to quantitatively evaluate the presence of IFN-τ during the critical period for maternal recognition of pregnancy in uterine flushings obtained in vivo by Foley catheter (Days 14, 16 and 18 post estrus) or post-mortem (Day 18 post estrus). Multiparous, cyclic or pregnant zebu cows (Bos taurus indicus) on days 14, 16 and 18 post estrus were used for in vivo or post mortem uterine flushing collection. In both cases, a Ringer solution was used to wash the uterus of cows. Uterine flushings were concentrated by ultra-filtration and lyophilized. Proteins were separated by one-dimensional electrophoresis (SDS-PAGE) in a 15% polyacrilamide gel. Interferon-tau quantification in uterine flushings was performed by western blotting and densitometry. Non-specific protein bands were observed in both in vivo and post mortem uterine flushings. Interferon-tau was detected only in uterine flushings obtained from pregnant cows post-mortem (P
Databáze: OpenAIRE
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