The Wnt signaling pathway regulates Nalm-16 b-cell precursor acute lymphoblastic leukemic cell line survival and etoposide resistance
| Autor: | Thiago, Leandro de Souza, Costa, Elaine Sobral da, Lopes, Daiana Vieira, Otazú, Ivone Beatriz, Nowill, Alexandre Eduardo, Mendes, F. A., Portilho, Márcio Figueiredo, Abreu Junior, Jose Garcia Ribeiro, Mermelstein, Claudia dos Santos, Orfao, A., Rossi, Maria Isabel Doria, Borojevic, Radovan |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Zdroj: | Repositório Institucional do INMETRO Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO) instacron:INMETRO |
| Popis: | Submitted by Jonathan Barbosa (jon_crf@hotmail.com) on 2012-02-14T15:50:15Z No. of bitstreams: 1 Borojevic2_2010.pdf: 1375185 bytes, checksum: fcf97c988706b7bfcad50dabccdf4f2a (MD5) Approved for entry into archive by Monica Couto(monicacbcouto@gmail.com) on 2012-06-19T18:07:25Z (GMT) No. of bitstreams: 1 Borojevic2_2010.pdf: 1375185 bytes, checksum: fcf97c988706b7bfcad50dabccdf4f2a (MD5) Made available in DSpace on 2012-06-19T18:08:47Z (GMT). No. of bitstreams: 1 Borojevic2_2010.pdf: 1375185 bytes, checksum: fcf97c988706b7bfcad50dabccdf4f2a (MD5) Previous issue date: 2010 B-cell precursor acute lymphoblastic leukemia (BCP-ALL) is the most common malignancy in children. The Wnt signaling pathway has been found to be extensively involved in cancer onset and progression but its role in BCP-ALL remains controversial.Weevaluate the role of the Wnt pathway in maintenance of BCP-ALL cells and resistance to chemotherapy. Gene expression profile revealed that BCP-ALL cells are potentially sensitive to modulation of Wnt pathway. Nalm-16 and Nalm-6 cell lines displayed low levels of canonical activation, as reflected by the virtually complete absence of total b-catenin in Nalm-6 and the b-catenin cell membrane distribution in Nalm-16 cell line. Canonical activation with Wnt3a induced nuclear b-catenin translocation and led to BCP-ALL cell death. Lithium chloride (LiCl) also induced a cytotoxic effect on leukemic cells. In contrast, both Wnt5a and Dkk-1 increased Nalm-16 cell survival. Also, Wnt3a enhanced the in vitro sensitivity of Nalm-16 to etoposide (VP-16) while treatment with canonical antagonists protected leukemic cells from chemotherapy-induced cell death. Overall, our results suggest that canonical activation of the Wnt pathway may exerts a tumor suppressive effect, thus its inhibition may support BCP-ALL cell survival. 10 p. : il. |
| Databáze: | OpenAIRE |
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