Eficácia de protocolos de medicação intracanal sobre o biofilme de Enterococcus faecalis em um modelo de dentes bovinos – estudo in vitro

Autor: Borba, Maristela Gutierrez de, Souza, Matheus Albino, Vier-Pelisser, Fabiana Vieira, Ghisi, Alexandre Correa, Nasário, Jéssia Stephanie Rodrigues, Oliveira, Silvia Dias de, Figueiredo, José Antônio Poli de
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Zdroj: Revista Odonto Ciência; Vol. 30 No. 2 (2015); 39-44
Revista Odonto Ciência; v. 30 n. 2 (2015); 39-44
Revista odonto ciência
Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron:PUC_RS
ISSN: 1980-6523
0102-9460
DOI: 10.15448/1980-6523.2015.2
Popis: Aim: The purpose of study was to evaluate, in vitro, the effectiveness of intracanal dressing protocols in root canals infected with E.faecalis. Methods: Eighty eight bovine incisors were contaminated with E. faecalis, remaining in culture for 30 days for biofilm formation. The teeth were divided into ten groups according to presence of disinfectant penetration (DP), intracanal dressing and medication placement site: G1 (CHX gel) – 2% chlorhexidine (CHX) gel (cervical third), G2 (CHX liq) – 2% CHX liquid (cervical third), G3 (TC) – tricresol formalin (canal entrance). In these groups (n=10), DP was not performed. G4 (DP+CHX gel) – 2% CHX gel (all thirds), G5 (DP+CHX liq) – 2% CHX liquid (all thirds), G6 (DP+TC) – tricresol formalin (canal entrance), G7 (DP+Ca(OH)2) – calcium hydroxide paste (all thirds). In these groups (n=10) DP with 2% NaOCl was performed. Groups G8 (DP NaOCl) – DP with 2% NaOCl, G9 (DP H2O) – DP with distilled water, and G10 – (no treatment) were considered controls (n=6). Microbiological test (CFUs counting) and scanning electron microscopy (SEM) were performed to evaluate and illustrate respectively the effectiveness of proposed treatments.Results: Microbiological test demonstrated that groups G4 (DP+CHX gel), G5 (DP+CHX liq), G6 (DP+TC) and G7 (DP+Ca(OH)2) showed no bacterial growth, being statistically different from all other groups (p
Databáze: OpenAIRE