Journal of Inorganic Biochemistry

Autor: Carneiro, Zumira Aparecida, Moraes, Juliana Cristina Biazzotto de, Rodrigues, Fernando Postalli, Lima, Renata Galvão de, Curti, Carlos, Rocha, Zênis Novaes da, Paulo, Michele, Bendhack, Lusiane Maria, Tedesco, Antonio Claudio, Formiga, André Luiz Barboza, Silva, Roberto Santana da
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Zdroj: Repositório Institucional da UFBA
Universidade Federal da Bahia (UFBA)
instacron:UFBA
ISSN: 0162-0134
DOI: 10.1016/j.jinorgbio.2011.04.011
Popis: texto completo: acesso restrito. p. 1035–1043. Submitted by Ana Valéria de Jesus Moura (anavaleria_131@hotmail.com) on 2012-02-13T20:28:40Z No. of bitstreams: 1 __pdn.sciencedirect.com_....0-S0162013411001073-main.pdf: 440339 bytes, checksum: c1ab70e5283b85632e2ac1a894823a34 (MD5) Made available in DSpace on 2012-02-13T20:28:40Z (GMT). No. of bitstreams: 1 __pdn.sciencedirect.com_....0-S0162013411001073-main.pdf: 440339 bytes, checksum: c1ab70e5283b85632e2ac1a894823a34 (MD5) Previous issue date: 2011 The synthesis, structural aspects, pharmacological assays, and in vitro photoinduced cytotoxic properties of [Ru(NO)(ONO)(pc)] (pc = phthalocyanine) are described. Its biological effect on the B16F10 cell line was studied in the presence and absence of visible light irradiation. At comparable irradiation levels, [Ru(NO) (ONO)(pc)] was more effective than [Ru(pc)] at inhibiting cell growth, suggesting that occurrence of nitric oxide release following singlet oxygen production upon light irradiation may be an important mechanism by which the nitrosyl ruthenium complex exhibits enhanced biological activity in cells. Following visible light activation, the [Ru(NO)(ONO)(pc)] complex displayed increased potency in B16F10 cells upon modifications to the photoinduced dose; indeed, enhanced potency was detected when the nitrosyl ruthenium complex was encapsulated in a drug delivery system. The liposome containing the [Ru(NO)(ONO)(pc)] complex was over 25% more active than the corresponding ruthenium complex in phosphate buffer solution. The activity of the complex was directly proportional to the ruthenium amount present inside the cell, as determined by inductively coupled plasma mass spectroscopy. Flow cytometry analysis revealed that the photocytotoxic activity was mainly due to apoptosis. Furthermore, the vasorelaxation induced by [Ru(NO)(ONO)(pc)], proposed as NO carrier, was studied in rat isolated aorta. The observed vasodilation was concentrationdependent. Taken together, the present findings demonstrate that the [Ru(NO)(ONO)(pc)] complex induces vascular relaxation and could be a potent anti-tumor agent. Nitric oxide release following singlet oxygen production upon visible light irradiation on a nitrosyl ruthenium complex produces two radicals and may elicit phototoxic responses that may find useful applications in photodynamic therapy.
Databáze: OpenAIRE
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