Genetic diversity and molecular survey of Trypanosoma (Megatrypanum ) theileri in cattle in Brazil’s western Amazon region
| Autor: | Pacheco,Thábata dos Anjos, Marcili,Arlei, Costa,Andrea Pereira da, Witter,Rute, Melo,Andréia Lima Tomé, Boas,Ricardo Vilas, Chitarra,Cristiane Silva, Dutra,Valéria, Nakazato,Luciano, Pacheco,Richard de Campos |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Revista Brasileira de Parasitologia Veterinária v.27 n.4 2018 Revista Brasileira de Parasitologia Veterinária Colégio Brasileiro de Parasitologia Veterinária (CBPV) instacron:CBPV |
| Popis: | Trypanosoma (Megatrypanum) theileri is a flagellated protozoan that infects ruminants and it displays high genetic diversity. In this study, we investigated the prevalence rates of this protozoan based on hemoculture and molecular diagnosis. The isolates of T. theileri thus obtained were characterized by molecular markers SSU rDNA and gGAPDH and molecular diagnosis based on Cathepsin L-like gene (PCR-TthCATL). The PCR-TthCATL and hemoculture indicated an overall prevalence rate of 8.13%, and the CATL derived sequence named IB was identified for the first time in cattle in the western Amazon region, as well as IF in Brazil. We also describe a possible new PCR-TthCATL derived sequence in cattle, designated IL. |
| Databáze: | OpenAIRE |
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