Potencial biotecnol??gico dos fungos endof??ticos do guaran?? (Paullinia cupana var. sorbilis) no controle biol??gico da antracnose

Autor: Bezerra, Thana Esashika
Přispěvatelé: Azevedo, Jo??o L??cio de, Pereira, Jos?? Odair, Galv??o, Rozana de Medeiros Sousa, Proc??pio, Rudi Emerson de Lima, Cavallazzi, Jos?? Renato Pereira
Jazyk: portugalština
Rok vydání: 2015
Předmět:
Zdroj: Biblioteca Digital de Teses e Dissertações da UFAM
Universidade Federal do Amazonas (UFAM)
instacron:UFAM
Popis: Submitted by Thana Esashika (thanaesashika@yahoo.com.br) on 2019-01-24T17:29:09Z No. of bitstreams: 3 Carta de aceite_doutorado.pdf: 310286 bytes, checksum: 07fb20b2f8b156c4026b01df534daf17 (MD5) Tese_Thana_23012019.pdf: 3834907 bytes, checksum: e132d006329364918f7fb1dcee3f0d1c (MD5) Ata_doutorado.pdf: 375153 bytes, checksum: 27f3a2f7f7863d29da6eb13c39baa8d5 (MD5) Approved for entry into archive by PPGBIOTEC Biotecnologia (ppg_biotec.ufam@yahoo.com.br) on 2019-01-24T20:14:40Z (GMT) No. of bitstreams: 3 Carta de aceite_doutorado.pdf: 310286 bytes, checksum: 07fb20b2f8b156c4026b01df534daf17 (MD5) Tese_Thana_23012019.pdf: 3834907 bytes, checksum: e132d006329364918f7fb1dcee3f0d1c (MD5) Ata_doutorado.pdf: 375153 bytes, checksum: 27f3a2f7f7863d29da6eb13c39baa8d5 (MD5) Rejected by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br), reason: A Carta de Encaminhamento anexada est?? desatualizada. Deve ser anexada a "Carta de Encaminhamento para o Autodep??sito" dispon??vel em http://biblioteca.ufam.edu.br/servicos/teses-e-dissertacoes D??vidas? ddbc@ufam.edu.br on 2019-01-24T20:20:42Z (GMT) Submitted by Thana Esashika (thanaesashika@yahoo.com.br) on 2019-01-24T20:39:30Z No. of bitstreams: 3 Tese_Thana_23012019.pdf: 3834907 bytes, checksum: e132d006329364918f7fb1dcee3f0d1c (MD5) Ata_doutorado.pdf: 375153 bytes, checksum: 27f3a2f7f7863d29da6eb13c39baa8d5 (MD5) CartaEncaminhamentoAutodep??sito_2019.pdf: 36552 bytes, checksum: 330d2131425e2eb957ad39d583557892 (MD5) Approved for entry into archive by PPGBIOTEC Biotecnologia (ppg_biotec.ufam@yahoo.com.br) on 2019-01-25T13:30:14Z (GMT) No. of bitstreams: 3 Tese_Thana_23012019.pdf: 3834907 bytes, checksum: e132d006329364918f7fb1dcee3f0d1c (MD5) Ata_doutorado.pdf: 375153 bytes, checksum: 27f3a2f7f7863d29da6eb13c39baa8d5 (MD5) CartaEncaminhamentoAutodep??sito_2019.pdf: 36552 bytes, checksum: 330d2131425e2eb957ad39d583557892 (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2019-01-25T14:46:27Z (GMT) No. of bitstreams: 3 Tese_Thana_23012019.pdf: 3834907 bytes, checksum: e132d006329364918f7fb1dcee3f0d1c (MD5) Ata_doutorado.pdf: 375153 bytes, checksum: 27f3a2f7f7863d29da6eb13c39baa8d5 (MD5) CartaEncaminhamentoAutodep??sito_2019.pdf: 36552 bytes, checksum: 330d2131425e2eb957ad39d583557892 (MD5) Made available in DSpace on 2019-01-25T14:46:27Z (GMT). No. of bitstreams: 3 Tese_Thana_23012019.pdf: 3834907 bytes, checksum: e132d006329364918f7fb1dcee3f0d1c (MD5) Ata_doutorado.pdf: 375153 bytes, checksum: 27f3a2f7f7863d29da6eb13c39baa8d5 (MD5) CartaEncaminhamentoAutodep??sito_2019.pdf: 36552 bytes, checksum: 330d2131425e2eb957ad39d583557892 (MD5) Previous issue date: 2015-06-26 FAPESP - Funda????o de Amparo ?? Pesquisa do Estado de S??o Paulo FAPEAM - Funda????o de Amparo ?? Pesquisa do Estado do Amazonas AMBEV - Companhia de Bebidas das Am??ricas 92991006450 The Amazon has a huge biological diversity, which is little known and consequently little explored, particularly with respect to microorganisms and their interactions with plants in the region. Among the microorganisms of interest are bacteria (including Actinomycetes) and fungi (including yeasts) that inhabit the interior and the surface of the host plants (endophytes and epiphytic microorganisms, respectively). Many of these microorganisms are potentially relevant characteristics as the production of antibiotics, flavorings, colorings, plant growth hormones and useful enzymes, among others. The aim of this study was to isolate, select, characterize and identify endophytic microorganisms present in the leaves, branches and rhizosphere of guarana (Paullinia cupana) as potential biocontrol agents of Colletotrichum. For this study, isolates of Colletotrichum were identified morphologically and molecularly and proven to be causative agents of anthracnose through the pathogenicity test. Then, the direct confrontation of 522 endophytic fungi against the pathogen Colletotrichum resulted in the selection of the following isolates showed significant antagonism rates: Penicillium verruculosum, Penicillium pinophilum, Geotrichum sp, Aspergillus flavus and Schizophyllum commune. Two in vivo experiments were performed in a greenhouse: For the first experiment, we used the five isolates in vivo test against Colletotrichujm gloeosporioides in which the fungi P. verruculosum, P. pinophilum, Geotrichum sp, A. flavus and. S. commune showed significant antagonistic effect on the fungus Colletotrichum gloeosporioides and none of them showed pathogenicity against P. cupana. The second experiment used two isolates of C. gloeosporioides, one in which the occurrence of double-stranded RNA bands (dsRNA) has been observed isolated, and the other without double-stranded RNA. For this experiment, it was found that the foliar lesions were lower in the leaves that were inoculated with the isolated double-stranded RNA, confirming thus a hypovirulent characteristic hipovirulenta isolated. In both experiments, it was observed that the endophytic fungi have the potential to control the pathogen plant pathogens in P. cupana and is promising for use in biocontrol of diseases of this plant. A Amaz??nia possui numa imensa diversidade biol??gica, a qual ?? pouco conhecida e por consequ??ncia pouco explorada, principalmente com rela????o aos microrganismos e as suas intera????es com as plantas da regi??o. Dentre os microrganismos de interesse est??o as bact??rias (incluindo os actinomicetos) e os fungos (incluindo as leveduras) que habitam o interior e a superf??cie das plantas hospedeiras (microrganismos endof??ticos e epif??ticos, respectivamente). Muitos destes microrganismos t??m potencialmente caracter??sticas relevantes como a produ????o de antibi??ticos, aromas, corantes, horm??nios de crescimento vegetal e enzimas ??teis, entre outras. O objetivo desta pesquisa foi isolar, selecionar, caracterizar e identificar microrganismos endof??ticos presentes nas folhas, ramos e rizosfera do guaran?? (Paullinia cupana) como poss??veis agentes de biocontrole da antracnose do guaranazeiro, causada por Colletotrichum gloeosporioides. Para esta pesquisa, Isolados de Colletotrichum gloeosporioides foram identificados morfol??gica e molecularmente e comprovados serem agentes causais da antracnose por meio do teste de patogenicidade. Em seguida, o confronto direto de 522 fungos endof??ticos contra o pat??geno C. gloeosporioides resultaram na sele????o dos seguintes isolados que apresentaram ??ndices de antagonismo significativos: Penicillium verruculosum, Penicillium pinophilum, Geotrichum sp., Schizophyllum commune e Aspergillus flavus. Foram realizados dois experimentos in vivo, na casa de vegeta????o: para o primeiro experimento, foram usados os cinco isolados no teste in vivo contra Colletottrichum gloeosporioides em que os fungos P. verruculosum, P. pinophilum, Geotrichum sp., A. flavus e S. commune apresentaram efeito antagonista significativo quanto ao fungo C. gloeosporioides e nenhum deles demonstrou patogenicidade contra P. cupana. O segundo experimento usou dois isolados de C. gloeosporioides, um no qual a ocorr??ncia de bandas RNA fita dupla (dsRNA) foi observada e o outro isolado, sem o RNA fita dupla. Para esse experimento, constatou-se que, as les??es foliares apresentaram-se menores nas folhas em que foi inoculado o isolado com o RNA fita dupla, constatando, assim, uma caracter??stica hipovirulenta do isolado. Em ambos os experimentos, observam-se que os fungos endof??ticos possuem potencial para o controle de fitopat??genos em P. cupana, sendo promissores para o uso no biocontrole de doen??as dessa planta.
Databáze: OpenAIRE
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