Análise estereológica e bioquímica do tecido conjuntivo e muscular no corpo cavernoso adjacente à placa fibrosa da doença de Peyronie

Autor: Ribeiro, Sabrina Barbosa Rebello Lessa
Přispěvatelé: Costa, Waldemar Silva, Sampaio, Francisco José Barcellos, Bastos, Ana Luiza, Favorito, Luciano Alves
Jazyk: portugalština
Rok vydání: 2008
Předmět:
Zdroj: Biblioteca Digital de Teses e Dissertações da UERJ
Universidade do Estado do Rio de Janeiro (UERJ)
instacron:UERJ
Popis: Submitted by Boris Flegr (boris@uerj.br) on 2021-01-07T22:27:46Z No. of bitstreams: 1 Sabrina Barbosa Rebello Lessa Ribeiro Dissertacao completa.pdf: 1018082 bytes, checksum: af11e9e7fa18b1f8c6d2f954d7754889 (MD5) Made available in DSpace on 2021-01-07T22:27:46Z (GMT). No. of bitstreams: 1 Sabrina Barbosa Rebello Lessa Ribeiro Dissertacao completa.pdf: 1018082 bytes, checksum: af11e9e7fa18b1f8c6d2f954d7754889 (MD5) Previous issue date: 2008-02-28 Studies on Peyronie disease (PD) usually focus the analysis on the fibrous plaque that forms in the tunica albuginea (TA). Because this fibrotic reaction is mediated by various inflammatory soluble factors adjacent connective tissues might also be affected. This secondary effect might explain, for example, the erectile dysfunction that occurs in this disease. We thus investigated the structural organization of the connective tissue in the corpus cavernosum (CC) adjacent to the fibrous plaque using stereological and biochemical techniques:During surgery of 7 PD patients (mean age 48.3 years), biopsies were obtained from the CC adjacent to the fibrous plaque and from the plaque itself. All patients had normal erection. Controls were similarly located samples from normal penises obtained during autopsy of 5 individuals (mean age 52.3 years). Tissue samples were stained with Weigert's stain (elastic fibers), Van Gieson's stain (connective tissue), and Sirius red (collagen). Stereological analysis was done using a 42-point grid to determine volumetric densities (Vv). Total collagen content was estimated as µg hyroxyproline per mg dry CC. Vv of elastic fibers was significantly reduced by 17.3% in PD compared with controls (19.49%±3.27% vs 23.56%±1.87%; p
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