Polyphasic characterization of endophytic bacteria of sorghum grown on cerrado soil of the Goiás State - Brazil

Autor: SILVA, L. B. da, SILVA, L. L. da, FERREIRA, E. P. de B., SANTOS, K. F. E. N., MARTIN-DIDONET, C. C. G.
Přispěvatelé: LARISSA BATISTA DA SILVA, UNIVERSIDADE ESTADUAL DE GOIÁS, LUCAS LEONARDO DA SILVA, UNIVERSIDADE ESTADUAL DE GOIÁS, ENDERSON PETRONIO DE BRITO FERREIRA, CNPAF, KARINA FREIRE D'EÇA NOGUEIRA SANTOS, UNIVERSIDADE ESTADUAL DE GOIÁS, CLAUDIA CRISTINA GARCIA MARTIN-DIDONET, UNIVERSIDADE ESTADUAL DE GOIÁS.
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice)
Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
Popis: Sorghum is a cereal of great commercial importance adapted to the tropical and subtropical regions. Isolation of plant-associated bacteria of agronomic interest has been the subject of several research works, however, there are few studies on bacteria isolating from sorghum. The objective of this work was to characterize as plant growth promoters endophytic bacteria isolated from sorghum roots. A total of 24 isolates were selected using the NFb-lactate, LGI-P, and YM semi-selective medium. The characterization was based on the resistance to antibiotics, use of carbon sources, enzyme activity and 16S-23S intergenic analysis. The isolates were more sensitive to the antibiotic streptomycin and showed a higher capacity to produce lipases and cellulases. Carbon sources of acid nature were more restrictive to the bacterial growth. Most of the isolates (95.8%) showed catalase and urease activity, besides to be able to solubilize phosphate. Intergenic analysis (16-23S rDNA) revealed a great diversity among the isolates, with eight groups with about 60% of similarity. In this study, it was possible to identify great physiological and genetic diversity among the bacteria isolated from sorghum roots. Some of these isolates can be considered promising plant growth-promoting rhizobacteria or sources of enzymes for biotechnological use. Made available in DSpace on 2017-10-26T09:35:22Z (GMT). No. of bitstreams: 1 CNPAF2017ijdr.pdf: 500717 bytes, checksum: 0bb1d02a2231c70a56abdf62ad178a3f (MD5) Previous issue date: 2017-10-25
Databáze: OpenAIRE