New approach for petroleum hydrocarbon degradation using bacterial spores entrapped in chitosan beads
| Autor: | Barreto, R.V.G., Hissa, Denise Cavalcante, Paes, Fernanda Araújo, Grangeiro, T. B., Nascimento, R. F., Rebelo, Luciana Magalhães, Craveiro, A. A., Melo, V. M. M. |
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| Jazyk: | portugalština |
| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | Repositório Institucional da Universidade Federal do Ceará (UFC) Universidade Federal do Ceará (UFC) instacron:UFC |
| Popis: | Spores ofBacillus subtilisLAMI008 were entrapped in 3-mm chitosan beads and cross-linked with 0.3%glutaraldehyde forn-hexadecane biodegradation and biosurfactant recovery. When exposed to nutrients,the spores generated vegetative cells without morphological alterations as revealed by atomic forcemicroscopy. The entrapped cells degraded almost 100% of 1% ofn-hexadecane in medium supplementedwith 1% glucose and produce biosurfactant within 48 h, as well as free cells. The number of viable cellsinside the beads was maintained throughout then-hexadecane degradation process and the released bio-surfactant was not used as a carbon source. Entrapment of bacterial spores in chitosan beads overcomesproblems with stability, storage, and long term cell viability encountered with vegetative cells. Thisapproach can potentially be utilized for biodegradation of complex compounds by entrapping sporesof different species of bacteria. |
| Databáze: | OpenAIRE |
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