Synergism between strains of a diesel-degrading bacterial consortium isolated from the Rio Negro

Autor: Santos, Jefferson Ferreira dos
Přispěvatelé: Andrade, Edmar Vaz de, Cordeiro, Isabelle Bezerra, Silva, Cynthia Can??do da, L??pez-Lozano, Jorge Luis, Pereira, Jos?? Odair, Carvalho, Elen Bethleen de Souza
Jazyk: portugalština
Rok vydání: 2021
Předmět:
Zdroj: Biblioteca Digital de Teses e Dissertações da UFAM
Universidade Federal do Amazonas (UFAM)
instacron:UFAM
Popis: Submitted by Jefferson Ferreira dos Santos (jfdsantos@ufam.edu.br) on 2021-12-16T16:35:53Z No. of bitstreams: 3 TESE_Versao_Final.pdf: 20076928 bytes, checksum: 5a49aa9aa97aa1c9d945783c8f421ab5 (MD5) Carta depo??sito da tese.pdf: 594219 bytes, checksum: f74d63c14d19ef4e37bfe897b3a99665 (MD5) Ata de defesa.pdf: 176065 bytes, checksum: 028492cdac68ac9944d2177bcce1ea0a (MD5) Approved for entry into archive by PPGBIOTEC Biotecnologia (ppgbiotecsecretaria@ufam.edu.br) on 2021-12-17T10:32:24Z (GMT) No. of bitstreams: 3 TESE_Versao_Final.pdf: 20076928 bytes, checksum: 5a49aa9aa97aa1c9d945783c8f421ab5 (MD5) Carta depo??sito da tese.pdf: 594219 bytes, checksum: f74d63c14d19ef4e37bfe897b3a99665 (MD5) Ata de defesa.pdf: 176065 bytes, checksum: 028492cdac68ac9944d2177bcce1ea0a (MD5) Rejected by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br), reason: Na Ficha Catalogr??fica as palavras-chave devem come??ar com a primeira letra mai??scula e as demais min??sculas, exceto quando forem siglas ou nomes pr??prios. on 2021-12-17T14:39:28Z (GMT) Submitted by Jefferson Ferreira dos Santos (jfdsantos@ufam.edu.br) on 2021-12-17T15:17:26Z No. of bitstreams: 3 Carta depo??sito da tese.pdf: 594219 bytes, checksum: f74d63c14d19ef4e37bfe897b3a99665 (MD5) Ata de defesa.pdf: 176065 bytes, checksum: 028492cdac68ac9944d2177bcce1ea0a (MD5) TESE_Versao_Final.pdf: 20170283 bytes, checksum: d4bbab95467dec07dfe1e10fbefc5247 (MD5) Approved for entry into archive by PPGBIOTEC Biotecnologia (ppgbiotecsecretaria@ufam.edu.br) on 2021-12-17T15:24:02Z (GMT) No. of bitstreams: 3 Carta depo??sito da tese.pdf: 594219 bytes, checksum: f74d63c14d19ef4e37bfe897b3a99665 (MD5) Ata de defesa.pdf: 176065 bytes, checksum: 028492cdac68ac9944d2177bcce1ea0a (MD5) TESE_Versao_Final.pdf: 20170283 bytes, checksum: d4bbab95467dec07dfe1e10fbefc5247 (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2021-12-17T18:00:28Z (GMT) No. of bitstreams: 3 Carta depo??sito da tese.pdf: 594219 bytes, checksum: f74d63c14d19ef4e37bfe897b3a99665 (MD5) Ata de defesa.pdf: 176065 bytes, checksum: 028492cdac68ac9944d2177bcce1ea0a (MD5) TESE_Versao_Final.pdf: 20170283 bytes, checksum: d4bbab95467dec07dfe1e10fbefc5247 (MD5) Made available in DSpace on 2021-12-17T18:00:28Z (GMT). No. of bitstreams: 3 Carta depo??sito da tese.pdf: 594219 bytes, checksum: f74d63c14d19ef4e37bfe897b3a99665 (MD5) Ata de defesa.pdf: 176065 bytes, checksum: 028492cdac68ac9944d2177bcce1ea0a (MD5) TESE_Versao_Final.pdf: 20170283 bytes, checksum: d4bbab95467dec07dfe1e10fbefc5247 (MD5) Previous issue date: 2021-12-10 FAPEAM - Funda????o de Amparo ?? Pesquisa do Estado do Amazonas Over the last few decades, pollution caused by hydrocarbons has been reported as one of the main environmental problems in the world. The sources of hydrocarbon contamination are as varied as possible, such as oil leaks, underground fuel tank leaks, waters contaminated by the production of oil and gas, among others. These activities can cause serious environmental and economic problems for terrestrial and marine ecosystems. The effects of these activities depend on the level of contamination, location of the accident and, above all, the environmental conditions at the time o the accident. Diesel is a fuel derived from oil and is among the most consumed of all fuels. In the Amazon region, its consumption is certainly higher due to the large amount of vessels powered by diesel oil, which can be an aggravating factor for environmental problems in the region. Alternatives that are able to promote the degradation of hydrocarbons, without further aggravating the environmental problem, is of extreme importance in the current scenario. Among the most used techniques in this decontamination process, bioremediation has gained prominence. There is, however, a consensus in the literature that no microorganism has the capacity to fully metabolize oil. Hence, the constant search for the isolation of consortia that promote the degradation of a greater amount of xenobiotics present in oil and its derivatives. Therefore, the objective of this work was to select a bacterial consortium capable of effectively degrading hydrocarbons present in diesel and then make a proteomic analysis of this selected bacterial consortium. The consortia were collected in the vicinity of the Port of Ceasa in the city of Manaus, Brazil. In total 5, consortia were collected, and all of them were able to use diesel as a carbon source, especially A3, which was superior to the others. The results presented for this consortium were the best for DCPIP reduction, emulsification (in xylene, hexane and diesel), cellular hydrophobicity (in xylene, hexane and diesel), diesel solubilization and in the toxicity test with L. sativa. The diesel degradation rate by the A3 consortium, in just 7 days, was higher than 70%. From the analysis of the proteome of this consortium, 890 proteins were identified. These proteins all belong to the species Acinetobacter baumannii, Burkholderia cenocepacia and Cupriavidus taiwanensis. Evidence was found for the degradation of 11 xenobiotic compounds present in diesel, distributed between aliphatic and aromatic compounds. The detailed analysis of the participation of proteins identified in the metabolic routes used by each species, enabled the proposition of a synergistic action between the three strains that make up the A3 consortium for the metabolism of diesel. Thus, the A3 consortium has a huge potential for the recovery of areas affected by diesel or other petroleum hydrocarbons. A polui????o causada pelos hidrocarbonetos tem sido relatada como um dos principais problemas ambientais no mundo h?? algumas d??cadas. As fontes de contamina????o por hidrocarbonetos s??o as mais variadas poss??veis, como vazamentos de ??leo, vazamentos de tanques de combust??vel subterr??neos, ??guas produzidas por petr??leo e g??s, entre outras. Essas atividades podem causar s??rios problemas ambientais e econ??micos para os ecossistemas terrestres e marinhos. Os efeitos dessas atividades dependem do volume de contamina????o, localiza????o do acidente e, principalmente, das condi????es ambientais da ??poca. O diesel ?? um combust??vel derivado do petr??leo e est?? entre os mais consumidos. Na regi??o amaz??nica, seu consumo ?? certamente mais elevado devido ?? grande quantidade de embarca????es movidas a ??leo diesel, o que pode ser um agravante para problemas ambientais na regi??o. Alternativas que sejam capazes de promover a degrada????o de hidrocarbonetos, sem agravar ainda mais o problema ambiental, ?? de extrema import??ncia diante do cen??rio atual. Entre as t??cnicas mais utilizadas nesse processo de descontamina????o, a biorremedia????o tem ganhado destaque. ?? consenso na literatura que nenhum microrganismo tem capacidade de metabolizar totalmente o petr??leo. Da?? a busca constante para o isolamento de cons??rcios que promovam a degrada????o de uma maior quantidade de xenobi??ticos presentes no petr??leo e seus derivados. Diante disso, o objetivo desse trabalho foi selecionar um cons??rcio bacteriano capaz de degradar efetivamente hidrocarbonetos presentes no diesel e em seguida fazer uma an??lise prote??mica desse cons??rcio bacteriano selecionado. Os cons??rcios foram coletados nas proximidades do porto do Ceasa na cidade de Manaus. No total 5 cons??rcios foram coletados, e todos eles se mostraram capazes de utilizar o diesel como fonte de carbono, em especial o A3, que se destacou em rela????o aos demais. Os resultados apresentados para esse cons??rcio foram os melhores para redu????o de DCPIP, emulsifica????o (em xileno, hexano e diesel), hidrofobicidade celular (em xileno, hexano e diesel), solubiliza????o do diesel e no teste de toxicidade em L. sativa. A taxa de degrada????o do diesel pelo cons??rcio A3, em apenas 7 dias, foi superior a 70 %. A partir da an??lise do proteoma desse cons??rcio foram identificadas 890 prote??nas pertencentes ??s esp??cies Acinetobacter baumannii, Burkholderia cenocepacia e Cupriavidus taiwanensis. Foram encontradas evid??ncias para degrada????o de 11 compostos xenobi??ticos presentes no diesel, distribu??dos entre compostos alif??ticos e arom??ticos. A an??lise detalhada da participa????o das prote??nas identificadas nas rotas metab??licas utilizadas por cada esp??cie, possibilitou a proposi????o de a????o sin??rgica entre as tr??s cepas constituintes do cons??rcio A3 para a metaboliza????o do diesel. Dessa forma, o cons??rcio A3 tem um enorme potencial para a recupera????o de ??reas afetadas por diesel ou outros hidrocarbonetos de petr??leo.
Databáze: OpenAIRE
Externí odkaz: