Journal of Clinical Mircobiology
| Autor: | Flannery, Brendan, Costa, Dirceu Joaquim, Carvalho, Fernanda Pinheiro, Guerreiro, Hygia, Matsunaga, James, Silva, Emilson Domingos Da, Ferreira, Antonio Gomes Pinto Ferreira |
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| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Zdroj: | Repositório Institucional da UFBA Universidade Federal da Bahia (UFBA) instacron:UFBA |
| DOI: | 10.1128/JCM.39.9.3303-3310.2001 |
| Popis: | Texto completo: acesso restrito. p. 3303-3310 Submitted by Suelen Reis (suelen_suzane@hotmail.com) on 2012-12-19T17:02:23Z No. of bitstreams: 1 3303.full.pdf: 214563 bytes, checksum: 5a395e3c74d15057c58cd36f8ebf3867 (MD5) Made available in DSpace on 2012-12-19T17:02:23Z (GMT). No. of bitstreams: 1 3303.full.pdf: 214563 bytes, checksum: 5a395e3c74d15057c58cd36f8ebf3867 (MD5) Previous issue date: 2001-09 There is an urgent need for development of new serodiagnostic strategies for leptospirosis, an emerging zoonosis with worldwide distribution. We have evaluated the diagnostic utility of five recombinant antigens in enzyme-linked immunosorbent assays (ELISAs) for serodiagnosis of leptospirosis. Sera from 50 healthy residents of a high-incidence region were used to determine cutoff values for 96% specificity. In paired sera from 50 cases of leptospirosis confirmed by the microscopic agglutination test, immunoglobulin G (IgG) but not IgM reacted with the recombinant leptospiral proteins. The recombinant LipL32 IgG ELISA had the highest sensitivities in the acute (56%) and convalescent (94%) phases of leptospirosis. ELISAs based on recombinant OmpL1, LipL41, and Hsp58 had sensitivities of 16, 24, and 18% during the acute phase and 72, 44, and 32% during convalescence, respectively. Compared to sera from healthy individuals, patient sera did not react significantly with recombinant LipL36 (P > 0.05). Recombinant LipL32 IgG ELISA demonstrated 95% specificity among 100 healthy individuals, and specificities ranging from 90 to 97% among 30 dengue patients, 30 hepatitis patients, and 16 patients with diseases initially thought to be leptospirosis. Among 39 Venereal Disease Research Laboratory test-positive individuals and 30 Lyme disease patients, 13 and 23% of sera, respectively, reacted positively with the rLipL32 antigen. These findings indicate that rLipL32 may be an useful antigen for the serodiagnosis of leptospirosis. |
| Databáze: | OpenAIRE |
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