Pharmacological effects of the ethanolic extract of the fruit peels of Nephelium lappaceum L. on nociception and gastric ulcer
| Autor: | Oliveira, Alan Santos |
|---|---|
| Přispěvatelé: | Camargo, Enilton Aparecido |
| Jazyk: | portugalština |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Repositório Institucional da UFS Universidade Federal de Sergipe (UFS) instacron:UFS |
| Popis: | Introduction: Rambutan, the fruit of Nephelium lappaceum L. (Sapindaceae) is popularly used to treat fever, stomach pain and diarrhea. The peels of rambutan contain a variety of phytochemicals, such as phenolic compounds, flavonoids and carotenoids, which add potential for several biological activities. Its use is suggested in cosmeceutical, nutraceutical and pharmaceutical preparations. Objective: The present study evaluated the antinociceptive and gastroprotective activities of the ethanolic extract of the fruit peels of N. lappaceum L. (EENL) in non-clinical models, as well as it evaluated whether this extract induces acute toxicity in zebrafish. Material and methods: EENL was characterized by chromatography coupled to mass spectrometry (RP-UHPLC-DAD). The experimental protocols were approved by the Animal Research Ethics Committee of Instituto Butantan – São Paulo (nº 6438210220) and UFS (nº 1122270819). An acute toxicity test was performed on zebrafish. Male Swiss mice were treated with EENL (50, 100 or 200 mg/kg, po, 1 hour before) and submitted to nociception assessment (abdominal writhing, paw formalin and capsaicin, carrageenan-induced mechanical hyperalgesia and hot plate test) and locomotor activity (open field). The ulcer model induced by acidified ethanol (60% ethanol/0.3 mol/L HCl) was used to evaluate the gastroprotective effect of EENL. In this model, the concentration of sulfhydryl compounds and lipid peroxidation in the gastric mucosa were also evaluated. The participation of non-protein sulfhydryl compounds (NP-SH), nitric oxide (NO), prostaglandins (PG), ATP-sensitive K+ channels (KATP) and hydrogen sulfide (H2S) in the gastroprotective effect of EENL was investigated in this model of ulcer using pharmacological tools. The effect of EENL on gastric volume, acidity and pH was evaluated in the pylorus ligation model, as well as mucus on gastric contents. Results: Procyanidin B, epicatechin, ellagic acid and their derivatives (such as punicalin and pedunculagin) were identified in the EENL. No toxicity was observed after incubating EENL with zebrafish embryos. Oral pretreatment of mice with EENL (200 mg/kg) did not alter locomotor activity, but pretreatment with doses of 50, 100 and 200 mg/kg reduced (p |
| Databáze: | OpenAIRE |
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