Bacteria isolated from abscesses of small ruminants inspected in the semiarid region of Brazil

Autor: SILVA, W. E. de L. e, GOLVEIA, G. V., SÁ, M. da C. A. de, GOUVEIA, J. J. de, PEIXOTO, R. de M., RIET-CORREA, F., VESCHI, J. L. A., COSTA, M. M. da
Přispěvatelé: WELLINGTON ERASMO DE LIMA E SILVA, Prof. Faculdade de Ciências Agrárias, FACIAGRA, Araripina, PE, GISELE VENERONI GOUVEIA, Prof. Universidade Federal do Vale do São Francisco, UNIVASF, MARIA DA CONCEIÇÃO AQUINO DE SÁ, JOÃO JOSÉ DE SIMONI GOUVEIA, Prof. Universidade Federal do Vale do São Francisco, UNIVASF, RODOLFO DE MORAES PEIXOTO, Prof. Instituto Federal do Sertão Pernambucano, IF SERTÃO, PE, Petrolina, PE, FRANKLIN RIET-CORREA, Prof. do Centro de Saúde e Tecnologia Rural, Universidade Federal de Campina Grande, UFCG, Patos, PB, JOSIR LAINE APARECIDA VESCHI, CPATSA, MATEUS MATIUZZI DA COSTA, Prof. Universidade Federal do Vale do São Francisco, UNIVASF, Petrolina, PE.
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice)
Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
Popis: Loss in the supply chain of small ruminants owing to condemnations of carcasses in the abattoirs and slaughterhouses is common in northeastern Brazil. This study aims to identify bacterial agents, including Mycobacterium spp., in the abscesses found in the postmortem analysis of the carcasses of sheep and goats bred in northeastern Brazil. Our analysis involved 679 goats and 1,838 sheep carcasses. Abscess samples were extracted and inoculated on blood agar and Lowenstein Jensen with pyruvate or glycerol for bacterial isolation. We then performed polymerase chain reaction of the hps 65 gene; samples positive for Mycobacterium spp. were subjected to DNA sequencing. Relative frequencies of abscesses in goats and sheep were 5.44 and 3.26%, respectively. Microbiological analysis revealed 87.7% bacterial growth in the inoculated samples. Among these, Corynebacterium pseudotuberculosisrepresented 67.7% of the isolates. We observed 1.9% mycobacteria growth in the abscess samples inoculated on Lowenstein-Jensen medium. PCR of DNA extracted from abscesses samples showed amplification of 0.9% of samples. After sequencing, Mycobacterium spp. isolate was identified as M. novocastrense. C. pseudotuberculosis was the main agent responsible for the formation of abscesses in the examined animals, and we did not identify any species of the M. tuberculosis complex in the examined small ruminants.
Databáze: OpenAIRE
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