Contributions in leaf morphoanatomy, phytochemistry, allelopathy and preliminary pharmacognostic characters of Piper marginatum Jacq. (Piperaceae), from Itacoatiara, Middle Amazon River

Autor: Nunes, Jander Savedra
Přispěvatelé: Campos, Ranieri, Guesdon, Isabel Reis, Abegg, Maxwel Adriano, Moura do Carmo, Dominique Fernandes
Jazyk: portugalština
Rok vydání: 2019
Předmět:
Zdroj: Biblioteca Digital de Teses e Dissertações da UFAM
Universidade Federal do Amazonas (UFAM)
instacron:UFAM
Popis: Submitted by Jander Savedra Nunes (jsavedranunes@hotmail.com) on 2019-09-04T08:32:10Z No. of bitstreams: 3 2.CartaEncaminhamentoAutodep??sito_Jander_Savedra_Nunes.pdf: 30980 bytes, checksum: 997190f3afef716287e7bd5b18b794df (MD5) 1.VERS??O_FINAL-PPGCTRA-Disserta????o_JANDER_SAVEDRA_NUNES_2019.pdf: 3288360 bytes, checksum: 690fc39d937c2b707e77bab1f0433a8e (MD5) 3.Ata de defesa P??blica_JANDER_SAVEDRA NUNES.pdf: 311665 bytes, checksum: d0153c44c0fcc65490aded6d7fcbfbe4 (MD5) Rejected by Renata Takeara (secretariappgctra@ufam.edu.br), reason: Prezado Senhor, sugerimos verificar a diferen??a entre o ti??tulo da disserta????o e o t??tulo que est?? grafado na ata da defesa e folha de aprova????o. Verifique poss??vle incorre????o e submeta novamente. Secretaria do PPGCTRA on 2019-09-04T14:31:28Z (GMT) Submitted by Jander Savedra Nunes (jsavedranunes@hotmail.com) on 2019-09-06T02:42:54Z No. of bitstreams: 3 1.VERS??O_FINAL-PPGCTRA-Disserta????o_JANDER_SAVEDRA_NUNES_2019.pdf: 3289268 bytes, checksum: d054293c57b1b1756418770d6a5387a1 (MD5) 2.CartaEncaminhamentoAutodep??sito_Jander_Savedra_Nunes.pdf: 30980 bytes, checksum: 997190f3afef716287e7bd5b18b794df (MD5) 3.Ata de defesa P??blica_JANDER_SAVEDRA NUNES.pdf: 311665 bytes, checksum: d0153c44c0fcc65490aded6d7fcbfbe4 (MD5) Rejected by Renata Takeara (secretariappgctra@ufam.edu.br), reason: Considere verificar o t??tulo do seu trabalho na carta de encaminhamento para autodep??sito e submeter novamente. on 2019-09-09T14:51:54Z (GMT) Submitted by Jander Savedra Nunes (jsavedranunes@hotmail.com) on 2019-09-09T15:46:58Z No. of bitstreams: 3 1.VERS??O_FINAL-PPGCTRA-Disserta????o_JANDER_SAVEDRA_NUNES_2019.pdf: 3289268 bytes, checksum: d054293c57b1b1756418770d6a5387a1 (MD5) 3.Ata de defesa P??blica_JANDER_SAVEDRA NUNES.pdf: 311665 bytes, checksum: d0153c44c0fcc65490aded6d7fcbfbe4 (MD5) 2.CartaEncaminhamentoAutodep??sito_Jander_Savedra_Nunes.pdf: 30801 bytes, checksum: 9eb92c753ecbb7593bf004bb0bcc981f (MD5) Approved for entry into archive by Renata Takeara (secretariappgctra@ufam.edu.br) on 2019-09-09T15:59:35Z (GMT) No. of bitstreams: 3 1.VERS??O_FINAL-PPGCTRA-Disserta????o_JANDER_SAVEDRA_NUNES_2019.pdf: 3289268 bytes, checksum: d054293c57b1b1756418770d6a5387a1 (MD5) 3.Ata de defesa P??blica_JANDER_SAVEDRA NUNES.pdf: 311665 bytes, checksum: d0153c44c0fcc65490aded6d7fcbfbe4 (MD5) 2.CartaEncaminhamentoAutodep??sito_Jander_Savedra_Nunes.pdf: 30801 bytes, checksum: 9eb92c753ecbb7593bf004bb0bcc981f (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2019-09-11T15:59:12Z (GMT) No. of bitstreams: 3 1.VERS??O_FINAL-PPGCTRA-Disserta????o_JANDER_SAVEDRA_NUNES_2019.pdf: 3289268 bytes, checksum: d054293c57b1b1756418770d6a5387a1 (MD5) 3.Ata de defesa P??blica_JANDER_SAVEDRA NUNES.pdf: 311665 bytes, checksum: d0153c44c0fcc65490aded6d7fcbfbe4 (MD5) 2.CartaEncaminhamentoAutodep??sito_Jander_Savedra_Nunes.pdf: 30801 bytes, checksum: 9eb92c753ecbb7593bf004bb0bcc981f (MD5) Made available in DSpace on 2019-09-11T15:59:12Z (GMT). No. of bitstreams: 3 1.VERS??O_FINAL-PPGCTRA-Disserta????o_JANDER_SAVEDRA_NUNES_2019.pdf: 3289268 bytes, checksum: d054293c57b1b1756418770d6a5387a1 (MD5) 3.Ata de defesa P??blica_JANDER_SAVEDRA NUNES.pdf: 311665 bytes, checksum: d0153c44c0fcc65490aded6d7fcbfbe4 (MD5) 2.CartaEncaminhamentoAutodep??sito_Jander_Savedra_Nunes.pdf: 30801 bytes, checksum: 9eb92c753ecbb7593bf004bb0bcc981f (MD5) Previous issue date: 2019-08-12 92991179098 Plant knowledge has always been useful to humanity, which is increasingly technological, has deepened its studies of plant resources to be sustainable. Bioactive plant phytochemicals are a promising source to be explored, and among them, many plant compounds of the genus Piper (Piperaceae) have been identified. Piper has ecological, economic and therapeutic importance. Its about 2,000 species have complex taxonomy, and errors in identification and use are common. Therefore, there is a need for a better characterization of the species of this genus. Piper marginatum Jacq. is widely used in traditional medicine. Among its classes of metabolites are terpenoids, phenylpropanoids and flavonoids, possessing properties like antifungal and antimicrobial, however there are no studies in allelopathy. This study investigated morphological and histochemical patterns of P. marginatum leaf and its phytochemical, allelopathic and antibacterial potentials. P. marginatum leaves were collected and fixed in the field and submitted to morphological and histochemical analysis, following classical methods. Extract from the leaves and their fractions were obtained to evaluate the phytochemical profile by colorimetric assays and in High Performance Liquid Chromatography. The allelopathic potential of the extract and fractions was investigated by measures of germination and growth, and antibacterial activity by disk diffusion. P. marginatum has typicall cordate shape with ciliated margin composed by tector trichomes, and the presence of two types of secretory idioblasts seems to be potential traits to their differentiation. These morphoanatomical characters has taxonomical potential and reinforce their proximity with other species of the genus. The histochemical results proved the prodution of phenolic compunds, alkaloids, polysaccharides and essential oils in the P. marginatum leaves. The crude extract (EB) yielded 3.03%; hexane fraction (FH): 31.13%, chloroform fraction (FC): 1.25%, and ethyl acetate fraction (FA): 0.97%. In phytochemistry were found phenolic compounds, flavonoids, tannins, anthocyanidins and phytosterols. Proven allelopathic activity of the crude extract and fractions significantly influenced the Germination Speed Index and the growth of the tested species, especially at concentrations of 500 ??g/mL. The strains of bacteria tested were not sensitive to the samples of this work. The flavonoid vitexin was identified. Thus, the contributions of this work are a potential aid to taxonomy and phytochemistry, as well as a significant parameter for future studies on allelopathy and other biological activities in P. marginatum. Additionally, botanical findings and pharmacognostic data provide parameters for the quality control of phytotherapic potentials, as well as being able to assist in studies of safe use of medicinal plants by native peoples. O conhecimento de plantas sempre foi ??til ?? humanidade, que cada vez mais tecnol??gica, tem apronfundado seus estudos dos recursos vegetais para ser sustent??vel. Os fitoconstituintes bioativos de plantas constituem uma fonte promissora a ser explorada, e entre eles, muitos compostos de plantas do g??nero Piper (Piperaceae) t??m sido identificados. Piper tem import??ncia ecol??gica, econ??mica e terap??utica. Suas cerca de 2.000 esp??cies tem taxonomia complexa, e erros de identifica????o e uso s??o comuns. Por isso h?? necessidade de melhor caracteriza????o das esp??cies desse g??nero. Piper marginatum Jacq. ?? uma esp??cie de Piper amplamente usada na medicina tradicional. Entre suas classes de metab??litos est??o terpenoides, fenilpropanoides e flavonoides, possuindo propriedades como anfif??ngica e antimicrobiana, por??m n??o h?? estudos em alelopatia. Este estudo investigou os padr??es morfol??gicos e histoqu??micos da folha de P. marginatum e seus potenciais fitoqu??mico, alelop??tico e antibacteriano. Folhas de P. marginatum foram coletadas e fixadas em campo e submetidas a an??lises morfoanat??micas e histoqu??micas, seguindo m??todos cl??ssicos. Extrato das folhas e suas fra????es foram obtidos para avaliar o perfil fitoqu??mico por ensaios colorim??tricos e por cromatografia ?? l??quido de alta efici??ncia. O potencial alelop??tico do extrato e fra????es foi investigado por medidas de germina????o e crescimento, e a atividade antibacteriana por difus??o em disco. P. marginatum possui um formato cordado t??pico com margem ciliada composta por tricomas tectores, e a presen??a de dois tipos de idioblastos secretores parece ser uma caracter??stica potencial para sua diferencia????o. Esses caracteres morfoanat??micos t??m potencial taxon??mico e refor??am sua proximidade com outras esp??cies do g??nero. Os resultados histoqu??micos comprovaram a produ????o de compostos fen??licos, alcaloides, polissacar??deos e ??leos essenciais nas folhas de P. marginatum. O extrato bruto (EB) rendeu 3,03%; fra????o hexano (FH): 31,13%, fra????o clorof??rmio (FC): 1,25%, e fra????o acetato de etila (FA): 0,97%. Na fitoqu??mica foram encontrados compostos fen??licos, flavonoides, taninos, antocianidinas e fitoester??is. Comprovada atividade alelop??tica do extrato bruto e fra????es influenciaram significativamente o ??ndice de Velocidade de Germina????o e o crescimento das esp??cies testadas, especialmente nas concentra????es de 500 ??g/mL. As cepas de bact??rias testadas n??o foram sens??veis ??s amostras deste trabalho. O flavonoide vitexina foi identificado. Com isso, as contribui????es deste trabalho consistem em um potencial aux??lio ?? taxonomia e ?? fitoqu??mica, al??m de significativo par??metro para futuros estudos em alelopatia e outras atividades biol??gicas em P. marginatum. Adicionalmente os achados bot??nicos e dados farmacogn??sticos fornecem par??metros para o controle de qualidade de potenciais fitoter??picos, al??m de poder servir de aux??lio em estudos de uso seguro de plantas medicinais por povos nativos. Parece faltar alguns bot??es de ajudas instant??neas como dicas "mais visuais"; Ser?? que ap??s um dep??sito o orientador recebe todas as c??pias para confirmar? Seria interessante ele responder com um c??digo de confirma????o; do tipo c??digo de barra.
Databáze: OpenAIRE
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