Brazilian Journal of Microbiology
| Autor: | Oliveira, Eugênia Márcia de Deus, Rodriguez, César Alejandro Rosales, Rocha, Vivianne Cambuí Mesquita, Ambrosio, Simone Rodriguez, Ohara, Patrícia Miyuki, Amaku, Marcos, Ferreira, Fernando |
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| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: | |
| Zdroj: | Repositório Institucional da UFBA Universidade Federal da Bahia (UFBA) instacron:UFBA |
| DOI: | 10.1590/S1517-83822007000400019 |
| Popis: | p. 687-692 Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2012-10-22T14:13:25Z No. of bitstreams: 1 __www.scielo.br_pdf_bjm_v38n4_a19v38n4.pdf: 146191 bytes, checksum: a0d3383dcb6c5264b8d49fc5337ef540 (MD5) Made available in DSpace on 2012-10-22T14:13:25Z (GMT). No. of bitstreams: 1 __www.scielo.br_pdf_bjm_v38n4_a19v38n4.pdf: 146191 bytes, checksum: a0d3383dcb6c5264b8d49fc5337ef540 (MD5) Previous issue date: 2007 Swine mycobacteriosis is an important cause of carcass condemnation at abattoirs. One of the best ways to recognize the etiologic agent involved, in live animals, is the fecal isolation, as 94% of the lesions are located in the digestive tract. Therefore, the goal of the present study was to compare the performance of four decontamination methods followed by inoculation in three different culture media, totalizing twelve procedures of mycobacteria search from swine fecal samples experimentally contaminated. The swine feces were artificially contaminated with 0.02 g of Mycobacterium avium, PIG-B strain, and subjected to mycobacteria isolation trial. The protocols used were: 1) modified Petroff or basic method; 2) modified Lowenstein-Jensen or acidic method; 3) modified Petroff or basic method with re-suspension in Amphotericin B; 4) modified Lowenstein-Jensen or acid method with re-suspension in Amphotericin B, followed by inoculation in Petragnani, Lowenstein-Jensen and Lowenstein-Jensen medium with antibiotics (Penicillin G and Nalidixic acid). There was a difference (p |
| Databáze: | OpenAIRE |
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