Maternal obesity increases lipogenesis and nonalcoholic fatty liver disease in the offspring until the F2 generation
| Autor: | Lannes, Wilian Rodrigues |
|---|---|
| Přispěvatelé: | Mandarim-de-Lacerda, Carlos Alberto, Sampaio, Francisco José Barcellos, Silva, Sandra Barbosa da, Torres, Thiago da Silva |
| Jazyk: | portugalština |
| Rok vydání: | 2015 |
| Předmět: |
Fat diet
Esteatose hepática - Aspectos nutricionais Gravidez - Aspectos nutricionais Fenômenos Fisiológicos da Nutrição Pré-Natal Genética Intergenerational Obesidade - Prevenção Intergeracional Metabolic programming Programação metabólica Nutrição - Aspectos genéticos Dieta hiperlipídica CIENCIAS DA SAUDE::NUTRICAO [CNPQ] Efeitos Tardios da Exposição Pré-Natal Fisiopatologia |
| Zdroj: | Biblioteca Digital de Teses e Dissertações da UERJ Universidade do Estado do Rio de Janeiro (UERJ) instacron:UERJ |
| Popis: | Submitted by Boris Flegr (boris@uerj.br) on 2021-01-05T18:14:14Z No. of bitstreams: 1 DISSERTACAO_FINAL_PUBLICADA_Wilian Rodrigues Lannes.pdf: 1562891 bytes, checksum: 9a9aa8464ae51811bde0394cf51a6064 (MD5) Made available in DSpace on 2021-01-05T18:14:14Z (GMT). No. of bitstreams: 1 DISSERTACAO_FINAL_PUBLICADA_Wilian Rodrigues Lannes.pdf: 1562891 bytes, checksum: 9a9aa8464ae51811bde0394cf51a6064 (MD5) Previous issue date: 2015-08-26 Conselho Nacional de Desenvolvimento Científico e Tecnológico Diet-induced maternal obesity may program susceptibility in offspring to chronic diseases and affect subsequent generations. The present study evaluated the effects of maternal obesity upon liver structure in early adult life, focusing on the F1 and F2 generations. C57BL/6 female mice (F0) were fed standard chow (SC) or HF diet (8 weeks) prior to mating and during the gestation and lactation to provide the F1 generation (F1-SC and F1-HF). At 3 months old, F1 females were mated to produce the F2 generation (F2-SC and F2-HF). The liver was dissected, weighed and sliced into several fragments at 5 μm thickness and stained with hematoxylin and eosin. Frozen liver tissue was used to analyze western blot and triacylglycerol. Data were analyzed by unpaired Student s t-test. The HF-F1 group had higher body mass (BM) at 90 days when compared with SC-F1 (P=0.001), whereas the HF-F2 offspring showed no significant difference in BM when compared to SC-F2 offspring. HF-F1 had glucose intolerance when compared to SC-F1 group. This result was not observed in the F2 offspring. Nevertheless, HF-F1 offspring (P=0.009) and the HF-F2 showed hyperinsulinemia (P=0.03). Regarding the hepatic steatosis, the HF-F1 offspring presented 18% more lipid droplets than SC-F1 offspring (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|