17β-estradiol alters endometrial protein expression in bovine females treated at the 17 th day of estrous cycle

Autor: Alves Jr, Sérgio Silva [UNESP], de Castro, Fernanda Cavallari [UNESP], Soares, Natália [UNESP], Pereira, Flávia Thomaz Verechia [UNESP], Balieiro, Júlio Cesar de Carvalho, Munin, Flávia Simone, Cesar, Marcelo de Cerqueira, Binelli, Mario, Membrive, Claudia Maria Bertan [UNESP]
Přispěvatelé: Universidade Estadual Paulista (Unesp), Universidade de São Paulo (USP)
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Zdroj: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Popis: Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:25:53Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:41:45Z : No. of bitstreams: 1 2-s2.0-79957550739.pdf: 4162045 bytes, checksum: 3acdb865a92019091a6c8a3bea947ee3 (MD5) Made available in DSpace on 2014-05-27T11:25:53Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-05-31 In bovine females the release of prostaglandin F 2α (PGF 2α) is induced in vivo by estradiol (E 2). It is believed that E 2 stimulates the synthesis of essential proteins for the production of PGF 2α. This study aimed to evaluate the effect of E 2 in increasing the concentration of total protein and modifying the protein composition of endometrial explants from bovine females treated with E 2 at the 17 th day of estrous cycle. Crossbred heifers were treated at 17 th day of estrous cycle intravenously with 0 mg (Control Group; n = 6) or 3 mg of E 2 (E 2 Group; n = 6) and killed two hours after. Endometrial explants were isolated, subjected to extraction of total protein, quantified and were analyzed by onedimensional electrophoresis on polyacrylamide gel 10% SDS-PAGE. The concentration of total protein did not differ between groups, 6296.10 + 439.90 μg/mL for the Control Group and 8426.56 + 1156.00 μg/mL for E 2 Group (p = 0.1158). There was no significant difference (p > 0.05) in the protein profile of endometrial explants in gels stained with Coomasie Blue. In gels stained with Silver Nitrate it was verified in E 2 Group greater relative percentage of the bands referring to the molecular weight of 75 to 76 kDa (8.40% vs. 4.89% in E 2 Group and Control respectively; p < 0.05) and 108 to 110 Kda (6.85% vs. 3.84% in E 2 Group and Control respectively, p < 0.05). It was observed in E 2 Group lower relative percentage of the band referring to the molecular weight of 90 kDa (5.78% vs. 9.83% in E 2 Group and control respectively; p < 0.05). We concluded that the E 2 does not increase the protein concentration in the endometrium, however, it modifies the proteinic composition in the endometrial explants, indicating that E 2 alters the expression of specific proteins. Faculdade de Odontologia Curso de Medicina Veterinária da Universidade Estadual Paulista, Araçatuba-SP Faculdade de Zootecnia Universidade Estadual Paulista Rodovia Comandante João Ribeiro de Barros (SP 294), Campus da UNESP, Dracena/SP, CEP 17900-000 Faculdade de Zootecnia Engenharia de Alimentos Da Universidade de São Paulo, Pirassununga-SP Faculdade de Medicina Veterinária Zootecnia Da Universidade de São Paulo, Pirassununga-SP Faculdade de Odontologia Curso de Medicina Veterinária da Universidade Estadual Paulista, Araçatuba-SP Faculdade de Zootecnia Universidade Estadual Paulista Rodovia Comandante João Ribeiro de Barros (SP 294), Campus da UNESP, Dracena/SP, CEP 17900-000
Databáze: OpenAIRE
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