In vivo neutralization of the dendrotoxin-mediated neurotoxicity of black mamba venom by oligoclonal human IgG monoclonal antibodies
| Autor: | Laustsen, Andreas Hougaard, Karatt Vellatt, Aneesh, Masters, Edward W., Arias, Ana Silvia, Pus, Urska, Knudsen, Cecilie, Oscoz, Saioa, Slavny, Peter, Griffiths, Daniel T., Luther, Alice M., Leah, Rachael A., Lindholm, Majken, Lomonte, Bruno, Gutiérrez, José María, McCafferty, John |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Nature Communications, vol.9(3928), pp. 1-9. Kérwá Universidad de Costa Rica instacron:UCR |
| Popis: | The black mamba (Dendroaspis polylepis) is one of the most feared snake species of the African savanna. It has a potent, fast-acting neurotoxic venom comprised of dendrotoxins and α-neurotoxins associated with high fatality in untreated victims. Current antivenoms are both scarce on the African continent and present a number of drawbacks as they are derived from the plasma of hyper-immunized large mammals. Here, we describe the development of an experimental recombinant antivenom by a combined toxicovenomics and phage display approach. The recombinant antivenom is based on a cocktail of fully human immunoglobulin G (IgG) monoclonal antibodies capable of neutralizing dendrotoxin-mediated neurotoxicity of black mamba whole venom in a rodent model. Our results show the potential use of fully human monoclonal IgGs against animal toxins and the first use of oligoclonal human IgG mixtures against experimental snakebite envenoming. Novo Nordisk Foundation/[NNF16OC0019248]//Dinamarca Hørslev Foundation/[203866]//Dinamarca Oticon Foundation/[17-3802]//Dinamarca Universidad de Costa Rica - Instituto Clodomiro Picado/[]//Costa Rica UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP) |
| Databáze: | OpenAIRE |
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