Detección de secuencias nucleotídicas de Mycobacterium bovis a partir de adn de moco nasal de caprinos inoculados experimentalmente
| Autor: | Isaura Carolina Ramírez Casillas, Marco A. Santillán Flores, Beatriz Arellano Reynoso, Francisco Morales Álvarez, Víctor R. Tenorio Gutiérrez |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: | |
| Zdroj: | Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias INIFAP Redalyc-INIFAP |
| Popis: | Excretion of M. bovis through aerosols and their inhalation is considered the main route of transmission and infection in cattle. The traditional methods used for monitoring the elimination of the microorganism by the nasal route are the bacteriological culture and the radiometric method. Recently developed molecular biology methods have favored the fast and reliable diagnosis of mycobacteria in the fi eld. In the present work, the M-PCR technique was used to determine the time at which M. bovis DNA could be detected from the nasal mucus of 14 experimentally-infected goats. Samples were taken from the nasal cavity at different time intervals after the challenge, and bacteriological culture and DNA amplifi cation by M-PCR were carried out. Sixty days after challenge, the M-PCR techniques detected six out of 14 animals as positive and at day 90, these same six animals plus four more were detected as positive. No sample was positive to the bacteriological culture. It is known that the bacteriological isolation of mycobacteria is diffi cult and that the reduced number of viable bacteria in each sample infl uences the result of the diagnosis. In this work, the advantage of using the M-PCR technique in order to obtain positive results in just a short time was demonstrated, which favors a quick and timely identifi cation of the animals that represent a risk of infection in the herd and that could represent reservoirs of the disease. |
| Databáze: | OpenAIRE |
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