Study of Cryptosporidium in domiciled dogs and cats through microscopic and molecular diagnosis

Autor: Oliveira, Amanda Gleyce Lima de
Přispěvatelé: Bomfim, Teresa Cristina Bergamo do, Sudr?, Adriana Pittella, Santos, Helena L?cia Carneiro
Jazyk: portugalština
Rok vydání: 2017
Předmět:
Zdroj: Biblioteca Digital de Teses e Dissertações da UFRRJ
Universidade Federal Rural do Rio de Janeiro (UFRRJ)
instacron:UFRRJ
Popis: Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2022-08-05T16:06:54Z No. of bitstreams: 1 2017 - Amanda Gleyce Lima de Oliveira.pdf: 2313140 bytes, checksum: c2a13f8484d09fe4df3fa446a23a9bc0 (MD5) Made available in DSpace on 2022-08-05T16:06:54Z (GMT). No. of bitstreams: 1 2017 - Amanda Gleyce Lima de Oliveira.pdf: 2313140 bytes, checksum: c2a13f8484d09fe4df3fa446a23a9bc0 (MD5) Previous issue date: 2017-05-31 The cryptosporidiosis is a intestinal and respiratory infection caused by parasites protists of the genus Cryptosporidium that infect a wide host range in all classes of vertebrates. The importance of transmission routes in the epidemiology of cryptosporidiosis is not totally clear, however, in the last decade several techniques of molecular biology have been developed to diagnose species of Cryptosporidium, genotypes and subtypes. These techniques are being used for epidemiological studies in endemic and epidemic areas, which has significantly improved the understanding of the transmission of cryptosporidiosis in humans and animals. In this context, the present study had the following objectives: to diagnose the presence of Cryptosporidium spp. oocysts by parasitological examination of faeces of dogs and cats domiciled; genotyping the species of the genus through nucleotide sequencing and performing phylogenetic analyzes, comparing the sequences obtained with those sequences deposited in GenBank. In total, 119 fecal samples were collected from residences belonging to the Campo Grande neighborhood, West Zone of the city of Rio de Janeiro, Brazil. Cryptosporidium oocysts were detected in the samples of 5/64 (7.8%) dogs and 3/55 (5.4%) cats. After the sequencing, C. canis (n = 3) and C. parvum (n = 2) were identified in dogs and C. felis (n = 3) was detected in cats. The three species diagnosed in this study are considered of importance in human and animal health. Besides cryptosporidiosis is debilitating for the host, these data reflect concern, because the species diagnosed have a zoonotic potential. In addition to the importance of the diagnosis of Cryptosporidium species in this study, another gastrointestinal parasites were detected and should not be neglected, since they are also important in both human and animal health. The results obtained in this study constituted the first registry of parasitism by C. parvum in dogs confirmed in Brazil and consequently in the state of Rio de Janeiro through the use of molecular tools, contributing to trace the molecular epidemiology of this etiological agent worldwide A criptosporidiose ? uma infec??o intestinal e respirat?ria causada por Protistas parasitos do g?nero Cryptosporidium que infectam uma ampla variedade de hospedeiros em todas as classes de vertebrados. A import?ncia das vias de transmiss?o na epidemiologia da criptosporidiose n?o ? totalmente clara, entretanto, na ?ltima d?cada v?rias t?cnicas de biologia molecular foram desenvolvidas para diagnosticar esp?cies de Cryptosporidium, gen?tipos e subtipos. Estas t?cnicas est?o sendo utilizadas para estudos epidemiol?gicos em ?reas end?micas e epid?micas, o que melhorou significativamente a compreens?o da transmiss?o de criptosporidiose em seres humanos e animais. Neste contexto, o presente estudo teve como objetivos: diagnosticar por meio do exame parasitol?gico de fezes de c?es e gatos domiciliados a presen?a de oocistos de Cryptosporidium spp.; caracterizar genotipicamente as esp?cies do g?nero atrav?s do sequenciamento nucleot?dico e realizar an?lises filogen?ticas, comparando as sequ?ncias obtidas com aquelas sequ?ncias depositadas no GenBank. No total, 119 amostras fecais foram coletas de resid?ncias pertencentes ao bairro de Campo Grande, Zona Oeste do munic?pio de Rio de Janeiro, Brasil. Oocistos de Cryptosporidium foram detectados nas amostras de 5/64 (7,8%) c?es e 3/55 (5,4%) gatos. Ap?s a realiza??o do sequenciamento, C. canis (n = 3) e C. parvum (n = 2) foram ?s esp?cies identificadas em c?es e C. felis (n = 3) foi detectada em gatos. As tr?s esp?cies diagnosticadas neste estudo s?o consideradas de import?ncia em sa?de humana e animal. Al?m da criptosporidiose ser debilitante para o hospedeiro, esses dados refletem preocupa??o, pois as esp?cies diagnosticadas, apresentam potencial zoon?tico. Al?m da import?ncia do diagn?stico de esp?cies de Cryptosporidium neste estudo, outros parasitos gastrointestinais foram detectados e n?o devem ser negligenciados, visto que tamb?m apresentam import?ncia tanto em sa?de humana quanto animal. Os resultados obtidos neste estudo constitu?ram primeiro registro de parasitismo por C. parvum em c?es confirmado no Brasil e consequentemente, no estado do Rio de Janeiro atrav?s do uso de ferramentas moleculares, contribuindo para tra?ar a epidemiologia molecular deste agente etiol?gico mundialmente
Databáze: OpenAIRE
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