Avaliação da eficácia dos diluidores tris ou água de coco em pó (ACP-106®), associado à Aloe vera (Aloe barbadensis Miller), na conservação de sêmen canino

Autor: MELO, Cibele Cavalcanti Souza de
Přispěvatelé: GUERRA, Maria Madalena Pessoa, OLIVEIRA, Érika Christina Santos, SILVA, Sildivane Valcácia, SILVA JUNIOR, Valdemiro Amaro da, SILVA, Ellen Cordeiro Bento da
Jazyk: portugalština
Rok vydání: 2015
Předmět:
Zdroj: Biblioteca Digital de Teses e Dissertações da UFRPE
Universidade Federal Rural de Pernambuco (UFRPE)
instacron:UFRPE
Popis: Submitted by (edna.saturno@ufrpe.br) on 2016-06-13T13:41:47Z No. of bitstreams: 1 Cibele Cavalcanti Souza de Melo.pdf: 1262965 bytes, checksum: a0b751f71b7ca0dbc7f0a215b3c2b60f (MD5) Made available in DSpace on 2016-06-13T13:41:47Z (GMT). No. of bitstreams: 1 Cibele Cavalcanti Souza de Melo.pdf: 1262965 bytes, checksum: a0b751f71b7ca0dbc7f0a215b3c2b60f (MD5) Previous issue date: 2015-02-27 Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq The aim was to evaluate the effect of the Aloe vera gel (Aloe barbadensis Miller), in association with the Tris base (hydroxymethyl aminomethane) or powdered coconut water (ACP- 106®) in canine semen conservation, as well as the action of this gel in the renewal process of the extender. Semen samples from five dogs, of breed Basset Hound, were used. In Experiment 1, samples were diluted in duplicate, using Tris plus 20% egg yolk (G1 and G2) or 5% Aloe vera (G3 and G4) and evaluated at 0, 48, 72 and 96 hours. After 48 hours, all samples were centrifuged for 10 minutes (400g) in a cooled centrifuge (5 °C). In the groups G1 and G3 the supernatant was removed and a new extender was added. In the other groups (G2 and G4) pellets were re-diluted in the same supernatant, without renewal. For Experiment 2, samples were divided into two equal aliquots, according to the treatments (G1: Tris + 20% egg yolk, control G2: Tris + 5% Aloe vera; G3: ACP-106® + 5% Aloe vera) and evaluated at 0, 24, 48 and 72 hours after cooling. In both experiments were performed sperm kinetics and membrane integrity analysis (iMP). In Experiment 1 results the diluent renewal did not affect on any parameter analyzed in the group using egg yolk (G1), shown when comparing the two treatments of this substance. The G2 (without renewal) did not demonstrate a significant difference when compared to G1 (renewal). However, groups with Tris-Aloe vera (G3 and G4) were lower (P
Databáze: OpenAIRE
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