Expression of a methionine-rich storage albumin from the Brazil nut (Bertholletia excelsa H.B.K., Lecythidaceae) in transgenic bean plants (Phaseolus vulgaris L., Fabaceae)
| Autor: | Aragão, Francisco José Lima, Barros, Leila Maria Gomes, Grossi-de-Sá, Maria Fatima, Almeida, Elionor Rita Pereira de, Gander, Eugen Silvano, Rech Filho, Elibio Leopoldo |
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| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Zdroj: | Repositório Institucional da UCB Universidade Católica de Brasília (UCB) instacron:UCB |
| Popis: | Made available in DSpace on 2016-10-10T03:52:23Z (GMT). No. of bitstreams: 5 Expression of a methionine Rich Storage Albumin From the Brazil.pdf: 59368 bytes, checksum: 0cce291aa98e76c9e434f1ad837b9f9f (MD5) license_url: 52 bytes, checksum: 2f32edb9c19a57e928372a33fd08dba5 (MD5) license_text: 24372 bytes, checksum: 94b0a37ff5ec51de8c55507bff4a7ff9 (MD5) license_rdf: 24623 bytes, checksum: 378d22d8fe50e084ee2f354be78cbe62 (MD5) license.txt: 1887 bytes, checksum: 445d1980f282ec865917de35a4c622f6 (MD5) Previous issue date: 1999 Bean (Phaseolus vulgaris), an important component in the diet of people in developing countries, has low levels of the essential amino acid, methionine. We have attempted to correct this deficiency by introducing a transgene coding for a methionine-rich storage albumin from the Brazil nut via biolistic methods. The transgene's coding sequence was driven by a doubled 35S CaMV promoter and AMV enhancer sequences. The transgene was stable and correctly expressed in homozygous R2 to R5 seeds. In two of the five transgenic lines the methionine content was significantly increased (14 and 23%) over the values found in untransformed plants. Sim Publicado |
| Databáze: | OpenAIRE |
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