In vitro propagation and acclimatization of Cattleya walkeriana Gardner grown in alternative culture media

Autor: Vicente, Michele Cagnin
Přispěvatelé: Ara?jo, Jo?o Sebasti?o de Paula, P?go, Rog?rio Gomes, Videira, Sandy Sampaio
Jazyk: portugalština
Rok vydání: 2019
Předmět:
Zdroj: Biblioteca Digital de Teses e Dissertações da UFRRJ
Universidade Federal Rural do Rio de Janeiro (UFRRJ)
instacron:UFRRJ
Popis: Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2022-03-17T20:57:29Z No. of bitstreams: 1 2019 - Michele Cagnin Vicente.pdf: 1900974 bytes, checksum: 0c286d2d963a943a252f3247184160e3 (MD5) Made available in DSpace on 2022-03-17T20:57:29Z (GMT). No. of bitstreams: 1 2019 - Michele Cagnin Vicente.pdf: 1900974 bytes, checksum: 0c286d2d963a943a252f3247184160e3 (MD5) Previous issue date: 2019-06-26 CAPES - Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior CNPq - Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico Cattleya walkeriana Gardner is an orchid endemic to Brazil of high commercial value and much coveted by orchid growers. Extractive exploitation and the degradation of its natural habitat, coupled with the reproductive specificities of the family, are factors that place it among the flora species at risk of extinction in the wild. In this way, in vitro propagation proves to be a valuable tool to obtain seedlings, aiming at supplying the market and preserving the species. In this context we sought to evaluate the development of Cattleya walkeriana Gardner seedlings grown in different culture media in vitro propagation system and later the ex vitro performance by acclimatization in greenhouse. The work consisted of three experiments; in the first one, protocorms approximately 1.0 cm in length were subcultured for 240 days in the Murashige and Skoog culture media (1962) (MS); Knudson C (1946) modified by Morel (1965) (KC); Peters? Fertilizer (PE); Kristalon ? Orange Fertilizer (KL); Culture medium B&G Orchid?es? (BG). In the second experiment the seedlings from the treatments MS, PE, KL and BG of the first experiment were taken to acclimatization in greenhouse where they were inoculated with 1.0 ml of the suspension of Azospirillum brasilense SP 245 (BR 11005) (AZ); 1.0 ml of the suspension of Microvirga vignae BR 3299 (MV); 1.0 ml of distilled H2O, this being the control treatment (CO). They remained for a period of 120 days in acclimatization. And in the third experiment, in order to validate the results of the previous experiments, Cattleya walkeriana Gardner protocorms were subcultured for 240 days in vitro in the KL and BG culture media, which had the concentrations of sucrose and activated carbon equal. In the first experiment it was possible to conclude that the Knudson C (1946) modified by Morel (1965) medium is not suitable for in vitro propagation of Cattleya walkeriana seedlings under the conditions of this experiment. The B&G Orchid?es? supply medium and the Kristalon ? Orange fertilizer culture medium proved to be the most efficient in seedling development, in addition the Peters? fertilizer culture medium was also shown as a good substitute for the medium of Murashige and Skoog (1962). There was no positive and / or negative correlation between the pH of the culture media at the end of the subculture and the biometric variables evaluated. In the second experiment, it can be concluded that, when acclimatized, the seedlings from the B&G Orchid?es? supply medium were the ones that presented the best results independently of the inoculation factor, so it can be concluded that the culture medium interferes in the acclimatization and that the B&G Orchid?es? supply medium is the most indicated in the in vitro propagation of the Cattleya walkeriana Gardner seedlings. In the third experiment, both the in vitro and the acclimatization stages, the best results were observed for the seedlings from the cultivation in the B&G Orchid?es? supply culture medium, demonstrating the superiority of this medium in relation to the KristalonTM Orange based fertilizer medium in propagation of Cattleya walkeriana Gardner. Cattleya walkeriana Gardner ? uma orqu?dea end?mica do Brasil de alto valor comercial e muito cobi?ada pelos orquid?filos. A explora??o extrativista e a degrada??o do seu habitat natural atrelados ?s especificidades reprodutivas da fam?lia s?o fatores que a colocam entre as esp?cies da flora em risco de extin??o na natureza. Deste modo, a propaga??o in vitro se mostra como uma valiosa ferramenta para obten??o de mudas, visando o abastecimento do mercado e a preserva??o da esp?cie. Nesse contexto buscou-se avaliar o desenvolvimento de pl?ntulas de Cattleya walkeriana Gardner cultivadas em diferentes meios de cultura em sistema de propaga??o in vitro e posteriormente o desempenho das mesmas ex vitro pela aclimatiza??o em casa de vegeta??o. O trabalho foi constitu?do de tr?s experimentos, no primeiro, protocormos com aproximadamente 1,0 cm de comprimento foram subcultivados durante 240 dias nos meios de cultura Murashige e Skoog (1962) (MS); Knudson C (1946) modificado por Morel (1965) (KC); Fertilizante Peters? (PE); Fertilizante KristalonTM Laranja (KL); Meio de cultura suprimento B&G Orchid?es? (BG). No segundo experimento as pl?ntulas provenientes dos tratamentos MS, PE, KL e BG do primeiro experimento foram levadas para aclimatiza??o em casa de vegeta??o onde foram inoculadas com 1,0 ml da suspens?o de Azospirillum brasilense SP 245 (BR 11005) (AZ); 1,0 ml da suspens?o de Microvirga vignae BR 3299 (MV); 1,0 ml de H2O destilada, sendo este o tratamento controle (CO). As mesmas permaneceram pelo per?odo de 120 dias em aclimatiza??o. E no terceiro experimento, com o objetivo de validar os resultados dos experimentos anteriores, protocormos de Cattleya walkeriana Gardner foram subcultivados durante 240 dias in vitro nos meios de cultura KL e BG, estes tiveram as concentra??es de sacarose e de carv?o ativado igualados. No primeiro experimento foi poss?vel concluir que o meio Knudson C (1946) modificado por Morel (1965) n?o ? adequado para propaga??o in vitro de pl?ntulas de Cattleya walkeriana sob as condi??es deste experimento. O meio de cultura suprimento B&G Orchid?es? e o meio de cultura a base de fertilizante KristalonTM Laranja se mostraram os mais eficientes no desenvolvimento das pl?ntulas, adicionalmente o meio de cultura a base de fertilizante Peters? tamb?m se mostrou como boa alternativa em substitui??o ao meio de cultura Murashige e Skoog (1962). N?o houve correla??o positiva e/ou negativa entre o pH dos meios de culturas ao final do subcultivo e as vari?veis biom?tricas avaliadas. No segundo experimento, pode-se concluir que quando aclimatizadas, as mudas provenientes do meio de cultura suprimento B&G Orchid?es? foram as que apresentaram os melhores resultados independentemente do fator inocula??o, logo, pode-se concluir que o meio de cultura interfere na etapa de aclimatiza??o e que o meio de cultura suprimento B&G Orchid?es? ? o mais indicado na propaga??o in vitro das pl?ntulas de Cattleya walkeriana Gardner. No terceiro experimento, tanto na etapa in vitro quanto na etapa de aclimatiza??o os melhores resultados foram observados para as pl?ntulas provenientes do cultivo no meio de cultura suprimento B&G Orchid?es?, comprovando a superioridade deste meio em rela??o ao meio a base de fertilizante KristalonTM Laranja na propaga??o de Cattleya walkeriana Gardner.
Databáze: OpenAIRE
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