Organ-related cigarette smoke-induced oxidative stress is strain-dependent
| Autor: | Barroso, Carlos Romualdo Rueff, Trajano, Eduardo Tavares Lima, Alves, Jackson Nogueira, Paiva, Rojane Oliveira, Lanzetti, Manuella, Pires, Karla Maria Pereira, Bezerra, Frank Silva, Pinho, Ricardo Aurino, Valenca, Samuel Santos, Porto, Luís Cristovão de Moraes Sobrino |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | Repositório Institucional da UFOP Universidade Federal de Ouro Preto (UFOP) instacron:UFOP |
| Popis: | Background: Cigarette smoke (CS) is associated with oxidative stress in several organs because it contains high concentrations of free radicals and reactive oxygen species. Experimental models, using different strains, provide important insights into the genetic basis of diseases. This study sought to identify, in different mouse strains, the organ that is most-susceptible to CS-induced oxidative stress to obtain an optimized experimental animal model of oxidative injury induced by CS. Material/Methods: Male Swiss, DBA/2, C3H, BALB/c, and C57BL/6 mice were exposed to CS 3 times a day (4 cigarettes per session) for 60 consecutive days. Control groups from the same strains were sham-treated. Protein content, malondialdehyde level, myeloperoxidase activity, and nitrite level were assayed in lung, liver, kidney, and brain from all strains. Catalase and glutathione peroxidase activities were measured. Analyses of data were done by using a 1-way ANOVA with Bonferroni’s post-test (P |
| Databáze: | OpenAIRE |
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