Caracteriza??o morfol?gica, aspectos biol?gicos e patogenia das formas evolutivas de Babesia bovis (Babes, 1888) e Babesia bigemina (Smith & Kilborne,1893) (Protozoa: babesiidae) em Boophilus microplus (Canestrini, 1887)
| Autor: | Barreira, Jairo Dias |
|---|---|
| Přispěvatelé: | Massard, Carlos Luiz, Rezende, Hugo Edison Barboza de, Freire, Nicolau Mau?s da Serra |
| Jazyk: | portugalština |
| Rok vydání: | 1988 |
| Předmět: | |
| Zdroj: | Biblioteca Digital de Teses e Dissertações da UFRRJ Universidade Federal Rural do Rio de Janeiro (UFRRJ) instacron:UFRRJ |
| Popis: | Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2020-10-26T16:42:20Z No. of bitstreams: 1 1988 - Jairo Dias Barreira.pdf: 728769 bytes, checksum: e943583f9dc8f048c7c260d0d41fb509 (MD5) Made available in DSpace on 2020-10-26T16:42:20Z (GMT). No. of bitstreams: 1 1988 - Jairo Dias Barreira.pdf: 728769 bytes, checksum: e943583f9dc8f048c7c260d0d41fb509 (MD5) Previous issue date: 1988-03-15 Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq The development of Boophilus microplus, Babesia bigemina and Babesia bovis was studied in experimental conditions in cattle raized in tick free area. The evolution of these blood parasites was followed in the tick vector, starting by the presence of infected erythrocytes in the intestinal lumen of engorged females, 24 hours after the ticks had dropped from the cattle host up to the detection of sporozoits in hatched larvae. During the period of 24 to 48 hours after engorged ticks dropped from the host it was observed the presence of infected erythrocytes as well as the presence of rayed bodies "Strahlenkorper" and vermiform bodies which looklike ookinete, result of gametogony. Ookineto were found in the cytoplasm of epithelial cells 72 hours after ticks hod neopped, as well as large number of immature sporozoites. During the some period of time the presence of immature and mature sporozoites of B. bigemina and B. bovis were found in hemolymph and in hemocytes. The presence of xxii. sporozoites in samples of malpighi tubes and ovaries were noticed at day 4. Eggs examined in the fourth and fifth day after engorged ticks had dropped were found to be positives for the presence of both species of Babesia. The ideal period for the diagnostic of B. bigemina and B. bovis in the hemolymph and in eggs of ticks was found to be at the 8th and 13th day after females dropped from the host, respectively. The examination of 100 engorged females of B. microplus from cattle which had a parasitemia of 1.5% for B. bigemina and 0.4% for B. bovis were found an infection rate of 96%. A second sample of females which dropped from cattle with a parasitemia of 1.8% for B. bigemina and 0.7% for B. bovis, had and infection rate of 88%. The evolution of B. bigemina and B. bovis in the invertebrate host affect the survival of engorged females. The mortality rate of engorged females observed seven and twelve days after dropping from the host fot the first and second groups were 94.79% and 75.0%, respectively. O desenvolvimento de Boophilus microplus, Babesia bigemina e B. bovis foi estudado em condi??es experimentais, usando bovinos mesti?os originados de uma regi?o livre destes parasitos. Observou-se uma sequ?ncia evolutiva dos hemoparasitos no carrapato vetor, partindo da presen?a de eritr?citos infectados na luz intestinal de tele?ginas, durante as primeiras 24 horas ap?s o desprendimento, at? o aparecimento de esporozoitas nas larvas. No per?odo de 24 a 48 horas ap?s a queda de f?- meas ingurgitadas, verificou-se al?m da presen?a de alguns eritr?citos infectados, a ocorr?ncia de corpos raiados "Strahlenkorper" e de formas vermiformes e que por seus aspectos morfol?gicos poderiam ser chamadas de oocinetos, podendo portanto estarem relacionados com a ocorr?ncia de gametogonia. Por volta de 72 horas ap?s a queda das tele?ginas, foi observado a presen?a de oocinetos no citoplasma das c?lulas epiteliais al?m de um grande n?mero de esporozoitas imaturos. No mesmo per?odo xx. foi observado a presen?a de esporozoitas imaturos e maturos de B. bigemina e B. bovis nas amostras de hemolinfa, e no interior dos hem?citos. A partir do quarto dia de incuba??o verificouse tamb?m, a presen?a dos esporozoitas em amostras de tubos de malpighi e ov?rio. Estes protozo?rios foram observados em amostras de ovos a partir do quarto e quinto dia ap?s a queda natural das tele?ginas, e em larvas com dois dias p?s-eclos?o. Concluiu-se que o per?odo ideal para o diagn?stico das infec??es por B. bigemina e B. bovis em amostras de hemolinfa e ovos corresponde ao oitavo e 13? dia ap?s a queda natural do hospedeiro vertebrado. Comprovou-se a interfer?ncia da infec??o por B. bigemina e B. bovis em rela??o ao p?so e postura das tele?ginas ap?s desprenderem-se naturalmente do hospedeiro. Do total de 100 tele?ginas coletadas de bovinos com parasitemia de 1,5% para B. bigemina e 0,4% para B. bovis, 96% apresentaram-se infectadas. Para o segundo grupo de tele?ginas ingurgitadas em bovinos com parasitemia de 1,8% para B. bigemina e 0,7% para B. bovis, a taxa de infec??o observada foi de 88%. A taxa de mortalidade observada no primeiro e segundo grupo de tele?ginas, durante o s?timo e o 12? dia, de incuba- ??o foi de 94,79 e 75,0%, respectivamente |
| Databáze: | OpenAIRE |
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