Genetic and phenotypic analysis of capuchin monkeys from Ilha da Marambaia, Mangaratiba, Rio de Janeiro

Autor: Penedo, Diego Mattos
Přispěvatelé: Nogueira, Denise Monnerat, NOGUEIRA, Denise Monnerat, ARMADA, Jorge Lu?s Azevedo de, GOLDSCHMIDT, Beatriz, SILVA, Heriberto Dias da, LOPES, Claudia Andrea de Araujo
Jazyk: portugalština
Rok vydání: 2016
Předmět:
Zdroj: Biblioteca Digital de Teses e Dissertações da UFRRJ
Universidade Federal Rural do Rio de Janeiro (UFRRJ)
instacron:UFRRJ
Popis: Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-02-22T20:14:46Z No. of bitstreams: 1 2016 - Diego Mattos Penedo.pdf: 4081734 bytes, checksum: 3e13fd4d6f8b8c4d8d31a05fefa2ba14 (MD5) Made available in DSpace on 2017-02-22T20:14:46Z (GMT). No. of bitstreams: 1 2016 - Diego Mattos Penedo.pdf: 4081734 bytes, checksum: 3e13fd4d6f8b8c4d8d31a05fefa2ba14 (MD5) Previous issue date: 2016-02-26 CAPES Several studies have been conducted with capuchin monkeys (Sapajus and Cebus) in order to understand their evolutionary histories by South and Central America. Genetic analysis, in association with the coat coloration, assist in the identification of the relationships between species and show the diversity presented by both genera. In Brazil, Sapajus species are predominantly distributed, being S. nigritus endemic to the Atlantic Forest and characteristic of southeast. At Ilha da Marambaia, an important area with forest remaining in the state of Rio de Janeiro, there is a population of capuchin monkeys, but without previous study. The objective of this study was to characterize the population of capuchin monkeys at this region through phenotypic and genetic analyzes, including the coat coloration, the sequencing of the mitochondrial gene cytochrome oxidase II and the evaluation of karyotype with conventional staining and C-band. Interviews with residents and military personnel in the region, were also carried out, in order to identify the possible origin and isolation of the primates. Twelve specimens, eight males and four females, were analyzed. The coat features were consistent with that described for S. nigritus, with coloration varying from brown to blackish on the back, limbs, tail and top of the head, in addition to yellow chest and face with white or light yellow, contrasting. Tufts at the top of the head were seen in adults, being greater in females. The analysis of mitochondrial gene revealed closer proximity of this population with S. xanthosternos, which occurs at Bahia, and then with S. cay that occurs in parts of Brazil and Paraguay. The greatest divergence was in relation to the population of S. nigritus in Argentina. The karyotype was consistent with that described for Sapajus species, with 2n = 54 (XX or XY), although the morphology of the sex chromosome Y, submetacentric, was different from that commonly described in the literature (acrocentric). Presented intercalary C-band the chromosome pairs 4, 11, 12 and 17. The par 11 presented three polymorphisms, all interstitial to euchromatin and diversified from each other by deletion and inversion processes. The pattern of this pair is different from that described, as a small acrocentric, to S. nigritus in populations of Argentina, with banding similar to that considered specific for S. xanthosternos. According to the survey, the population of primates seems to be native of the island and is currently isolated. According to the genetic divergences found, the population of Ilha da Marambaia may have maintained ancestral characteristics. The C-band pattern of the pair 11 indicates that the polymorphism described for populations of Argentina does not correspond to the entire distribution of S. nigritus. These data may help to understand the diversity and evolution of Sapajus, since this would have radiated from southeastern Brazil. Further studies with populations from other regions of Rio de Janeiro are needed to verify the C-band pattern presented for the pair 11 as well as the genetic relationships demonstrated by the mitochondrial gene for the population of Ilha da Marambaia. Diversos estudos v?m sendo realizados com macacos-prego e caiararas (Sapajus e Cebus) de modo a entender suas hist?rias evolutivas pelas Am?ricas do Sul e Central. An?lises gen?ticas, em associa??o ?s caracter?sticas de pelagem, auxiliam na identifica??o das rela??es entre as esp?cies e evidenciam a diversidade apresentada por ambos os g?neros. No Brasil, est?o distribu?das predominantemente as esp?cies de Sapajus, sendo S. nigritus end?mica de Mata Atl?ntica e caracter?stica do sudeste. Na Ilha da Marambaia, importante ?rea com remanescente florestal no estado do Rio de Janeiro, existe uma popula??o de macacos-prego, sem nenhum estudo anterior. O objetivo deste trabalho foi caracterizar a popula??o de macacos-prego dessa regi?o atrav?s de an?lises fenot?picas e gen?ticas, incluindo o padr?o de pelagem, o sequenciamento do gene mitocondrial Citocromo Oxidase II e a avalia??o do cari?tipo com colora??o convencional e bandamento de heterocromatina constitutiva (banda C). Foram realizadas, tamb?m, entrevistas com moradores e militares da regi?o, de modo a identificar a poss?vel origem e isolamento dos primatas. Foram analisados doze esp?cimes, oito machos e quatro f?meas. As caracter?sticas de pelagem coincidiram, de modo geral, com descrito para S. nigritus, sendo observada colora??o variando de marrom a enegrecida no dorso, membros, cauda e topo da cabe?a, al?m de peito amarelo e face branca ou amarelo-clara, contrastante. Tufos de pelos no topo da cabe?a foram observados nos adultos, sendo maiores nas f?meas. A an?lise do gene mitocondrial revelou maior proximidade da popula??o da ilha com S. xanthosternos, que ocorre na Bahia, e em seguida com S. cay, que ocorre em parte do Brasil e no Paraguai. A maior diverg?ncia apresentada foi em rela??o a popula??es de S. nigritus da Argentina. A an?lise do cari?tipo revelou padr?o condizente com as esp?cies de Sapajus, com 2n = 54 (XX ou XY), embora a morfologia do cromossomo sexual Y, submetac?ntrica, fosse divergente do comumente descrito na literatura (acroc?ntrica). Apresentaram banda C intercalar os pares cromoss?micos 4, 11, 12 e 17. O par 11 mostrou-se com tr?s polimorfismos, todos intercalares e diversificados entre si por processos de dele??o e invers?o. O padr?o deste par foi divergente do descrito, como um pequeno acroc?ntrico, para S. nigritus em popula??es da Argentina, apresentando bandamento semelhante ao considerado espec?fico de S. xanthosternos. Segundo o levantamento realizado, a popula??o de primatas parece ser natural da ilha, estando provavelmente isolada. De acordo com as diverg?ncias gen?ticas encontradas, a popula??o da Ilha da Marambaia pode ter conservado caracter?sticas ancestrais. O padr?o de banda C do par 11 indica que o polimorfismo descrito para popula??es da Argentina pode n?o corresponder a toda a distribui??o de S. nigritus. Estes dados podem auxiliar no entendimento da diversidade e evolu??o das esp?cies de Sapajus, uma vez que este teria irradiado a partir do sudeste brasileiro. Novos estudos com popula??es de outras regi?es do Rio de Janeiro s?o necess?rios para se averiguar o padr?o de bandamento de heterocromatina apresentada para o par 11, bem como as rela??es gen?ticas demonstradas pelo sequenciamento do gene mitocondrial para a popula??o da Ilha da Marambaia.
Databáze: OpenAIRE
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