| Popis: |
Relevance of the problem and aim of the work. To create fully adequate restoration we must consider not only chemical composition of used materials, their treatment, but also their antibacterial features. Main materials used for prosthetics parts are titanium, zirconium oxide, polymethilmethacrylate (PMMA) and polyetheretherketone (PEEK). Aim of study: to investigate and evaluate the formation of colonies of bacteria P. gingivalis (causing peri-implant infection) on different implant abutment materials: titanium, zirconium oxide, PMMA and PEEK Materials and methods. In this study were four groups (n=10): titanium (Ti), zirconium oxide (ZrO2), PMMA and PEEK. All samples were treated with abrasive paper of silicon carbide and polishing paste. Roughness of surface (Ra) was examined using atomic force microscope (AFM). Bacteria P. gingivalis ATCC 33277 was used in this in vitro study to measure colony forming units per one mililiter (CFU/ml) on surface of each examined group. Data analysis were carried out using SPSS 22 application, by using Kruskal-Wallis, Mann-Whitney, Chi-Squere tests. Results. The examination of surface roughness (Ra) of samples revealed that it was lowest in ZrO2 (9,36 nm). The surface of Ti (22,05 nm) was two times rougher than of ZrO2. The highest level of Ra was found in PMMA group (62,33 nm). Ra of PEEK material = 49,57 nm. Statistically significant results of formation of P. gingivalis bacteria colonies were found between PMMA and all three examined materials: ZrO2, PEEK, Ti and between ZrO2 and PEEK groups. No statistically significant results were found between Ti and ZrO2. Surfaces roughness significantly correlated with biofilm formation on each group. Conclusions: The lowest amount of the bacteria P. gingivalis has been found on the titanium and zirconium oxide. The greatest growth of bacteria P. gigivalis colonies were on PMMA material. |
