| Zdroj: |
International Journal of Gynecological Cancer : 20th International Meeting of the European Society of Gynaecological Oncology : Vienna, Austria, November 4-7, 2017 : Meeting Abstracts / European Society of Gynaecology Oncology. International Gynecologic Cancer Society, Philadelphia : Lippincott Williams & Wilkins, 2017, vol. 27, suppl. 4, no. 1747-1747, no. ESGO7-1208 |
| Popis: |
Aims To detect low, medium and high-risk human papilloma virus (HPV) types in histological samples of cervical cone biopsies for carcinoma in situ (CIS) in order to evaluate different HPV types and other risk factors influence on development of cervical CIS. Method 100 formalin fixed and paraffin – embed histological samples of cervical CIS after cone biopsy were investigated for low, medium and high-risk HPV types, using specific set for HPV-DNA extraction from tissue and amplification using polymerase chain reaction (PCR). We evaluated 15 high-risk (16, 18, 31, 33, 35, 39, 45,51, 52, 56, 58, 59, 68, 73, 82), 3 possiblycarcinogenic (26, 53, 66) and 6 low-risk (6, 11, 42, 43, 44) HPV types. The influence of other factors on developing CIS was evaluated using an original questionnaire. Results Nine different HPV types were detected in 77% of samples. In 85.7% of samples – only one HPV type was detected, in 14.3% - two HPV types. HPV 16 was detected statistically more frequently in all samples (p=0.002) and this trend was seen in all age groups. The biggest HPV types variety was seen in 30 – 49 years old patients group. No significant differences between different HPV types combinations were found. The non-smokers were statistically older at the time of the cervical cone biopsy (p |
