| Autor: |
Babušytė, Agnė, Jeroch, Jolanta, Stakauskas, Rimantas, Stravinskaitė, Kristina, Malakauskas, Kęstutis, Sakalauskas, Raimundas |
| Jazyk: |
litevština |
| Rok vydání: |
2010 |
| Předmět: |
|
| Zdroj: |
Medicina, Kaunas : Kauno medicinos universitetas, 2010, t. 46, Nr. 5, p.315-322 |
| ISSN: |
1010-660X |
| Popis: |
Objective. The aim of study was to investigate a chemotactic effect of induced sputum and bronchoalveolar lavage fluid on blood neutrophils in patients with chronic obstructive pulmonary disease (COPD) and healthy individuals. Material and methods. Forty-three smokers with COPD, 19 ex-smokers with COPD, 13 healthy smokers, and 17 healthy nonsmokers were recruited to the study. Neutrophils were isolated from peripheral blood of study individuals. For the same experimental conditions, pooled induced sputum and bronchoalveolar lavage fluid of 20 COPD patients were used. Neutrophil chemotaxis in vitro was performed in cell-transmigration chamber. Substances tested for chemoattraction (interleukin-8, induced sputum, bronchoalveolar lavage fluid directly or in addition to interleukin-8) were added to lower wells. Upper wells were filled with 2.5×106/mL of neutrophil culture and incubated for 2 hours. Migration was analyzed by flow cytometry. Results. Interleukin-8 (10–100 ng/mL) induced a dose-dependant neutrophil migration in all the groups. Only 100 ng/L of interleukin-8 induced more intensive chemotaxis of neutrophils from COPD smokers as compared to ex-smokers (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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