Métabolomique non ciblée sur gouttes de sang séché
| Autor: | Lagrée, Marie, Mercier, Antoine, Pujos-Guillot, Estelle, Durand, Stéphanie |
|---|---|
| Přispěvatelé: | Plateforme Exploration du Métabolisme (PFEM), MetaboHUB-Clermont, MetaboHUB-MetaboHUB-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Réseau Francophone de Métabolomique et Fluxomique (RFMF) |
| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | 15èmes journées scientifiques du RFMF 15èmes journées scientifiques du RFMF, May 2023, Perpignan, France |
| Popis: | International audience; Blood micro-sampling was initially used in hospitals to screen newborns for congenital diseases; today, this type of sampling is increasingly used in clinical studies. Analysed mainly using targeted methods, few studies report results using an open metabolomics approach. The aim of the work presented here is to evaluate sampling devices, extraction methods for these samples and the analytical coverage obtained by LCHRMS. Whatman 903® collection papers, mainly used for these samples, and Mitra® Neoteryx supports with a fixed sample volume of 20µL, were tested under different storage and extraction conditions. Analyses were carried out using heparinised plasma and whole blood on LC-QToF, then reprocessed on W4M. Initial results enabled a qualitative comparison to be made between heparinised plasma samples and the same samples deposited on the media studied under the different conditions. After optimising the preparation of this type of sample, the whole blood samples could be characterised.In conclusion, this preliminary work in open metabolomics will make it possible to investigate the analysis of micro-samples, a minimally invasive collection method that is easy to use at home, to answer questions relating to clinical studies and the development of participatory science. |
| Databáze: | OpenAIRE |
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