Hypochlorous acid effects on volume regulation of migrating neutrophils
Autor: | Kuznetsova, T., Kulahava, T., Zholnerevich, I., Divakova, N., Nebieridze, M., Semenkova, G. |
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Jazyk: | ruština |
Rok vydání: | 2013 |
Zdroj: | Бюллетень сибирской медицины. |
ISSN: | 1682-0363 |
Popis: | Cell volume regulatory mechanisms participate in different activities of cells including neutrophils migration and ROS generation [1, 2]. The effect of HOCl on the random migration of human neutrophils was studied. Isolated neutrophils (untreated and HOCl-treated) were placed on glass for 20 min and fixed. The volume ( V ) and projection area ( S ) were calculated for single control ( n = 130) and treated cells ( n = 160) using atomic-force microscopy. Because neutrophils movement consists of repeated cycles including the formation of lamellipodium during cell spreading and a cell retraction, we have identified three cell groups according to its area: 1) S < 75 mm 2, inactive cells and hemispherical retractile cells; 2) S from 75 to 150 mm 2, cells with growing lamellipodium; 3) S > 150 mm 2, fully spreading cells. The average V of control cells in group 2 increased by 30% as compared to group 1 cells and the values of cell volume varied around a constant mean of 149 mm 3 (from 98 to 225 mm 3). As volume changes associated with lamellipodium growth, as individual size differences of neutrophils may cause this volume fluctuation. With a further area rise above 150 mm 2 the variability of volume values became very low (average V 155 mm 3, max V 220 mm 3). The rapid increasing of cell area led to marked S/V ratio changes. It varied from 0.4 to 1.8. The average V of exposed to HOCl cells from group 1 was less than control value (96 mm 3). With cell spreading it increased initially by 57%. A slow gradual increase of cell volume was observed for the entire further area growth from 75 mm 2 to the maximum. As a result the average V of treated cells reached to control value (group 2) and then overpassed (165 mm 3, group 3). The fluctuation of volume values was greater and persisted in group 3. Because cell area dynamics was similar to the control, the average ratio S / V of treated cells was lower. Thus, HOCl alters cell volume regulation of random migrating human neutrophils. |
Databáze: | OpenAIRE |
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