Действие селективного света на рост клеточных культур растения Artemisia annua L
| Jazyk: | ruština |
|---|---|
| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | Вестник Томского государственного университета. Биология. |
| ISSN: | 2311-2077 1998-8591 |
| Popis: | Изучено действие селективного света на рост каллусной и суспензионной культур Artemisia annua L. (полыни однолетней). Установлено, что зеленый свет вызывает замедление роста каллусной культуры Artemisia annua L., а также увеличение объемов клеток, что может свидетельствовать о замедлении процессов клеточного деления. Показано, что максимальных значений индекс роста достигал в каллусной культуре полыни однолетней, выращиваемой на синем свету. Выяснено, что красный свет достоверно замедляет рост суспензионной культуры Artemisia annua L. по сравнению с синим светом One of the major source of medicinal substances for human diseases curing is the plants. Artemisia annua L. (sweet wormwood) which are the origin of sesquiterpen lactone artemisinin and its derivatives with antimalarial, antiparasitic, antiviral and antitumour effects, is the exercise of such plant. Traditionally artemisinin has been made from intact plants. However, the content of this substance in various plant organs is nonuniform. Consequently, for increasing of sesquiterpene lactones supply on the world markets we must use cell culture technology. The process of creating this technology needed to obtain basic knowledge about the physiological regulation of biologically active substances content in the plant cell. Selective lighted controlling of morphogenesis, plant productivity and secondary metabolites biosynthesis is the one of the most important factors of this regulation. Thus, the aim of our work was researching a selective light effects on A. annua L. callus and suspension growth parameters. Growth of callus culture A. annua L. for subculture period in 15 days was studied. Maximum values of the growth index were on culture under blue light, while the green light suppressed ones. Maximum cell volume were in callus under green light, both red and blue leads to a reduction of this index, this can show increasing of proliferative activity under blue light. The effect of selective light on A. annua L. suspension growth for 10 days subculturing on NAA and BAP contained medium was studied. There are no significant differences between cell volumes on the selective light and control. Suspension culture under red light has reduced values of growth index and dry weight production in compare with culture under blue light. |
| Databáze: | OpenAIRE |
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