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The anthers from six pepper genotypes, two hybrids Edita F1, Homera F1, and 4 cultivars: Duga bela, Una, Amfora and Kurtovska kapija were used in two-year androgenesis experiment. The anthers were isolated and cultivated on suitable nutrient media, following appropriate protocols. All tested pepper genotypes responded differently in terms of callus and androgenic embryos formation. Four androgenic regenerants (Edita_R1, Edita_R2, Edita_R3 and Edita_R4) from the genotype Edita F1 were used for molecular evaluation and compared with the donor genotype. The DNA was extracted according to the CTAB protocol, modified by Somma (2004). SSR markers Hpms1-117, Hpms 1-168, Hpms 1-274, EPMS 650 and CAMS 117 were used for molecular analysis. The molecular evaluation of the regenerants showed that all androgenic regenerants had the same allele for all SSR loci as donor genotype Edita F1. Additionally, all androgenic regenerants were homozygotes for the five tested loci. |
