| Popis: |
Phosphorus is the second most important nutrient for plant growth, but its availability is very low in the soil. This study was designed to identify phosphate-solubilizing Rhizobia from rhizosphere soil in Gorogutu and Deder districts of Ethiopia. The study evaluates the effects of phosphate-solubilizing Rhizobia on Teff (Eragrostis teff) growth under greenhouse conditions. The greenhouse experiment was assembled in a completely randomized design with 5 treatments including the control with three replications. A total of 30 Rhizobia isolates were obtained from rhizosphere soils in two districts (Gorogutu, and Deder) and screened for phosphate solubilization using Pikovskaya’s Agar. Based on their phosphate solubilization indices, 4 potential isolates (PS-6, PS-22, PS-27, and PS-30) were selected for further study in the greenhouse. Phosphate solubilizing isolates in pot experiment under greenhouse were applied at a rate of 0.5 g per pot and Teff disinfected seeds were suspended in 30 ml thick cell suspension of phosphate solubilizing Rhizobia (1012cells/ml) in the presence of 0.5% peptone (antidiuretic hormone for 30 minutes), but not the control. The result showed that almost all inoculants, after 30- and 90-days inoculation, have significantly improved the growth parameters of Teff (Eragrostis teff) under greenhouse conditions. Moreover, all the isolates showed a positive impact on all the growth parameters. The results from this experiment are useful in Ethiopia for biofertilizer development and for teff growth under organic farming systems. Therefore, isolates PS-6 and PS-22 are highly recommended to be prospective commercial biofertilizers at field conditions in different teff agroecologies. |
