| Autor: |
Tůma, S., Izaguirre, Jon Kepa, Bondar, Maryna, Marques, Mafalda M., Fernandes, Pedro C.B., da Fonseca, M. M.R., Cesário, Maria Teresa |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Popis: |
Agar extraction from Gelidium and Gracilaria red seaweed species produces hundred thousand ton of carbohydrate-rich residues annually. Gelidium sesquipedale waste biomass obtained after agar extraction, still contained 44.2 % w/w total carbohydrates (dry-weight basis). These residues were biologically up-graded to poly-3-hydroxybutyrate (P3HB) after saccharification of their carbohydrate fraction to simple sugars. A combined hydrolysis treatment using sulfamic acid followed by enzymatic hydrolysis with cellulases produced a glucose-rich hydrolysate with a negligible content of inhibitors. With this treatment a sugar yield of circa 30 % (g glucose/g biomass) was attained. The algal hydrolysates were assessed as carbon source for the production of P3HB by the halotolerant bacteria Halomonas boliviensis. A cell concentration of 8.3 g L−1containing 41 % (w/w) of polymer and a yield (YP/S) of 0.16 gpolymer/gglucosewere attained in shake flask assays. In this work, cellulose-rich seaweed waste was shown to be an upgradable, sustainable source of carbohydrates. © 2020 |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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