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Dariusz Szala, Joanna Czech, Marzena Skrzypa, Marta Kopanska, Dorota Hanf-Osetek, Krzysztof Gargasz, GrzegorzGuzik, Slawomir Snela, Izabela Zawlik. MiRNA expression profile in serum of osteoarthritis patients. Journal of Education, Health and Sport. 2019;9(11):11-30. eISSN 2391-8306. DOI http://dx.doi.org/10.12775/JEHS.2019.09.11.001 https://apcz.umk.pl/czasopisma/index.php/JEHS/article/view/JEHS.2019.09.11.001 https://zenodo.org/record/3528173 The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019. © The Authors 2019; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike. (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 25.10.2019. Revised: 04.11.2019. Accepted: 04.11.2019. MiRNA expression profile in serum of osteoarthritis patients Dariusz Szala 3*, Joanna Czech 1,2*, Marzena Skrzypa 1,2*, Marta Kopanska 1,2, Dorota Hanf-Osetek 3, Krzysztof Gargasz 4, Grzegorz Guzik 6, SlawomirSnela3,5, Izabela Zawlik 1,2** 1Laboratory of Molecular Biology, Centre for Innovative Research in Medical and Natural Sciences, Faculty of Medicine, University of Rzeszow, Poland; 2Department of Genetics, Chair of Molecular Medicine, Faculty of Medicine, University of Rzeszow, Poland; 3Department of Orthopaedic and Traumatology, University Hospital No 2, Rzeszow, Poland; 4Data Analysis Laboratory, Centre for Innovative Research in Medical and Natural Sciences, Faculty of Medicine, University of Rzeszow, Poland; 5Institute of Physiotherapy, Faculty of Medicine, University of Rzeszow,Poland 6Department of Oncological Orthopaedics, Specialist Hospital in Brzozów – Podkarpacie Oncological Centre, Poland *Equal contribution **Corresponding author: tel. +48 17 851 68 10, izazawlik@yahoo.com Laboratory of Molecular Biology, Center for Innovative Research in Medical and Natural Sciences, Faculty of Medicine,University of Rzeszow, Warzywna 1A, 35-310 Rzeszow, Poland Abstract Introduction: Osteoarthritis (OA) is the most common form of arthritis that affects millions of people worldwide. Despite advances in medicine, OA still remains incurable. Scientists are still seeking candidates for biomarker-based risk stratification and the early detection of disease. Measurement of miRNAs in the serum may become a powerful tool in the development of diagnostic biomarker. Purpose:The aim of this study was to examine the expression profiles of 20 miRNAs in the serum of patients with OA. Methods:We used RT-qPCR method to measure the expression profile of selected miRNAs. Results:Six miRNAs showed differential expression between OA and normal serum samples. miR-146a-5p and miR-98-5p were significantly upregulated (P < 0.05), while miR-222-3p, miR-22-3p, miR-27a-3p and miR-93-5p were downregulated (P < 0.05) compared to the control group. To the best of our knowledge, this is the first work that shows that expression of these six miRNAs is different between serum OA samples and healthy controls. However, we did not find any statistically significant relationship between the miRNAs expression and clinical characteristics. Conclusions:Our findings suggest that miRNAs can be used as potential biomarkers for OA, but further research is needed to assess the usefulness of serum miRNAs in miRNA-based prognostic and therapeutic approaches. Key words:expression; miRNA; osteoarthritis; serum |
