Large-Scale Production of Lentiviral Vectors: Current Perspectives and Challenges
| Autor: | Martínez-Molina, Eduardo, Chocarro-Wrona, Carlos, Martínez-Moreno, Daniel, Marchal, Juan A., Boulaiz, Houria |
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| Přispěvatelé: | [Martínez-Molina,E, Chocarro-Wrona,C, Martínez-Moreno,D, Marchal,JA, Boulaiz,H] Biopathology and Medicine Regenerative Institute (IBIMER), University of Granada (D.M.), Granada, Spain. [Chocarro-Wrona,C, Boulaiz,H] Department of Human Anatomy and Embryology, University of Granada, Granada, Spain. [Chocarro-Wrona,C, Boulaiz,H] Excellence Research Unit 'Modeling Nature' (MNat), University of Granada, Granada, Spain. [Chocarro-Wrona,C, Boulaiz,H] Biosanitary Institute of Granada (ibs.GRANADA), SAS-Universidad de Granada, Granada, Spain., This research was supported by Fundacion Empresa Universidad de Granada (Project PR/18/001), Fundación Mutua Madrileña (project FMM-AP16683-2017), Consejería de Salud Junta de Andalucía (PI-0089-2017), from the Chair 'Doctors Galera-Requena in cancer stem cell research' and Consejería de Economía, Conocimiento, Empresas y Universidades de la Junta de Andalucía (SOMM17/6109/UGR, FEDER Funds). |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Bioreactor
Anatomy::Cells [Medical Subject Headings] Organisms::Viruses::RNA Viruses::Retroviridae::Lentivirus [Medical Subject Headings] Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Gene Transfer Techniques [Medical Subject Headings] Large-scale production Terapia genética Gene therapy Anatomy::Cells::Cells Cultured::Cell Line [Medical Subject Headings] Analytical Diagnostic and Therapeutic Techniques and Equipment::Equipment and Supplies::Bioreactors [Medical Subject Headings] Information Science::Information Science::Communications Media::Publications::Review Literature as Topic [Medical Subject Headings] Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Clinical Laboratory Techniques::Cytological Techniques::Cell Culture Techniques [Medical Subject Headings] Lentiviral vector Reactores biológicos Analytical Diagnostic and Therapeutic Techniques and Equipment::Therapeutics::Biological Therapy::Genetic Therapy [Medical Subject Headings] |
| Popis: | Lentiviral vectors (LVs) have gained value over recent years as gene carriers in gene therapy. These viral vectors are safer than what was previously being used for gene transfer and are capable of infecting both dividing and nondividing cells with a long-term expression. This characteristic makes LVs ideal for clinical research, as has been demonstrated with the approval of lentivirus-based gene therapies from the Food and Drug Administration and the European Agency for Medicine. A large number of functional lentiviral particles are required for clinical trials, and large-scale production has been challenging. Therefore, efforts are focused on solving the drawbacks associated with the production and purification of LVsunder current good manufacturing practice. In recent years, we have witnessed the development and optimization of new protocols, packaging cell lines, and culture devices that are very close to reaching the target production level. Here, we review the most recent, efficient, and promising methods for the clinical-scale production ofLVs. Yes |
| Databáze: | OpenAIRE |
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