Evolution of Experimental Models in the Study of Glioblastoma: Toward Finding Efficient Treatments
| Autor: | Gómez-Oliva, Ricardo, Domínguez-García, Samuel, Carrascal, Livia, Abalos-Martínez, Jessica, Pardillo-Díaz, Ricardo, Verástegui, Cristina, Castro, Carmen, Nunez-Abades, Pedro, Geribaldi-Doldán, Noelia |
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| Přispěvatelé: | [Gómez-Oliva,R, Domínguez-García,S, Abalos-Martínez,J, Pardillo-Díaz,R, Castro,C] Área de Fisiología, Facultad de Medicina, Universidad de Cádiz, Cádiz, Spain. [Gómez-Oliva,R, Carrascal,L, Verástegui,C, Castro,C, Nunez-Abades,P, Geribaldi-Doldán,N] Instituto de Investigación e Innovación Biomédica de Cádiz (INIBICA), Cádiz, Spain. [Carrascal,L, Nunez-Abades,P] Departamento de Fisiología, Facultad de Farmacia, Universidad de Sevilla, Sevilla, Spain. [Verástegui,C, Geribaldi-Doldán,N] Departamento de Anatomía y Embriología Humanas, Facultad de Medicina, Universidad de Cádiz, Cádiz, Spain, This work was supported by the Spanish Ministerio de Ciencia, Innovación y Universidades (Grant Numbers RTI2018–099908-B-C21) and co-financed by the 2014-2020 ERDF Operational Programme and by the Department of Economy, Knowledge, Business and University of the Regional Government of Andalusia. Project reference: FEDER-UCA18-106647. This work has been co-financed by the Integrated Territorial Investment Operational Programme of the European Commission and by the Department of Department of Health and Families (Consejería de Salud y Familias) of the Regional Government of Andalusia. Project reference: ITI-0042-2019 ITI CÁDIZ 2019. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Neoplasias encefálicas
Células madre Diseases::Nervous System Diseases::Central Nervous System Diseases::Brain Diseases::Brain Neoplasms [Medical Subject Headings] Anatomy::Cells::Stem Cells::Neoplastic Stem Cells [Medical Subject Headings] Cell cultures of glioma cells Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings] Brain organoids Analytical Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Cytological Techniques::Cell Culture Techniques [Medical Subject Headings] Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Models Theoretical [Medical Subject Headings] Mouse models of glioblastoma Organisms::Eukaryota::Animals [Medical Subject Headings] Glioma stem cells Phenomena and Processes::Physiological Phenomena::Pharmacological Phenomena::Drug Resistance [Medical Subject Headings] Anatomy::Body Regions::Transplants::Heterografts [Medical Subject Headings] Anatomy::Cells::Cellular Structures::Extracellular Space::Extracellular Matrix [Medical Subject Headings] 3D bioprinting Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice [Medical Subject Headings] Stem Cells Diseases::Neoplasms::Neoplasms by Histologic Type::Neoplasms Germ Cell and Embryonal::Neuroectodermal Tumors::Neoplasms Neuroepithelial::Glioma::Astrocytoma::Glioblastoma [Medical Subject Headings] Anatomy::Tissues::Organoids [Medical Subject Headings] Extracellular matrix Glioma Anatomy::Nervous System::Central Nervous System::Brain [Medical Subject Headings] Bioimpresión Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Bioprinting [Medical Subject Headings] Brain tumor Matriz extracelular Chemicals and Drugs::Pharmaceutical Preparations::Dosage Forms::Colloids::Suspensions [Medical Subject Headings] |
| Popis: | Glioblastoma (GBM) is the most common form of brain tumor characterized by its resistance to conventional therapies, including temozolomide, the most widely used chemotherapeutic agent in the treatment of GBM. Within the tumor, the presence of glioma stem cells (GSC) seems to be the reason for drug resistance. The discovery of GSC has boosted the search for new experimental models to study GBM, which allow the development of new GBM treatments targeting these cells. In here, we describe different strategies currently in use to study GBM. Initial GBM investigations were focused in the development of xenograft assays. Thereafter, techniques advanced to dissociate tumor cells into single-cell suspensions, which generate aggregates referred to as neurospheres, thus facilitating their selective expansion. Concomitantly, the finding of genes involved in the initiation and progression of GBM tumors, led to the generation of mice models for the GBM. The latest advances have been the use of GBM organoids or 3D-bioprinted mini-brains. 3D bio-printing mimics tissue cytoarchitecture by combining different types of cells interacting with each other and with extracellular matrix components. These in vivo models faithfully replicate human diseases in which the effect of new drugs can easily be tested. Based on recent data from human glioblastoma, this review critically evaluates the different experimental models used in the study of GB, including cell cultures, mouse models, brain organoids, and 3D bioprinting focusing in the advantages and disadvantages of each approach to understand the mechanisms involved in the progression and treatment response of this devastating disease. Yes |
| Databáze: | OpenAIRE |
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