| Popis: |
Diabetes is one of the major health problems worldwide with rapidly growing incidence. It is progressive disease characterized by the inability to produce or/and properly use insulin. Regardless of medical treatment, diabetic patients as well as animals have recurring episodes of hyperglycemia that permit the development of diabetic complications. Episodes of hyperglycemia after eating are particularly harmful. It was known that glucose, resulting from digestive process, goes across intestinal membrane and is absorbed in the blood stream. Partial reduction of glucose uptake in small intestine of diabetic organism might prevent hyperglycemia-induced stress and the development of diabetic complications. The use of in vivo and in vitro models in studying disease is fundamental to the advancement of understanding of mechanisms, disease dysfunctions and development and testing of therapeutics. The presentation will be focused on mouse models, which we have used in research with the special accent on a new method for analysis of diabetic/control urine. Also, we will present our preliminary results observed by using Caco-2 cell line. Namely, human intestinal cells have been extensively used to study absorption and intestinal physiology. Thereby, our attention will be focused on the investigation of reducing glucose uptake after cell treatment with Ca Zeolite A. |
