A direct mass spectrometry method for the rapid analysis of a ubiquitous tire derived toxin N-(1,3-dimethylbutyl)-N’-phenyl-p-phenylenediamine quinone (6-PPDQ)

Autor: Monaghan, Joseph, Jaeger, Angelina, Agua, Alon R., Stanton, Ryan S., Pirrung, Michael, Gill, Chris G., Krogh, Erik T.
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Popis: This is an accepted peer-reviewed manuscript of an article that was published as: Monaghan, J., Jaeger, A., Agua, A.R., Stanton, R.S., Pirrung, M., Gill, C.G., & Krogh, E.T. (2021). A direct mass spectrometry method for the rapid analysis of a ubiquitous tire derived toxin N-(1,3-dimethylbutyl)-N’-phenyl-p-phenylenediamine quinone (6-PPDQ). Environmental Science & Technology Letters, 8(12), 1051-1056. https://doi.org/10.1021/acs.estlett.1c00794 The oxidative transformation product of a common tire preservative, identified as N-(1,3-dimethylbutyl)-N’-phenyl-p-phenylenediamine quinone (6-PPDQ), has recently been found to contribute to ‘urban run-off mortality syndrome’ in Coho salmon at ng/L levels. Given the number of fish bearing streams with multiple stormwater inputs, large-scale campaigns to identify 6-PPDQ sources and evaluate mitigation strategies will require sensitive, high throughput analytical methods. We report the development and optimization of a direct sampling tandem mass spectrometry method for semi-quantitative 6-PPDQ determinations using a thin polydimethylsiloxane membrane immersion probe. The method requires no sample clean up steps or chromatographic separations, even in complex, heterogenous samples. Quantitation is achieved by the method of standard additions, with a detection limit of 8 ng/L and 15 min/sample duty cycle. High throughput screening provides semi-quantitative concentrations with similar sensitivity and a 2.5 minute/sample full analytical duty cycle. Preliminary data and performance metrics are reported for 6-PPDQ present in representative environmental and stormwater samples. The method is readily adapted for real-time process monitoring, demonstrated by following 6-PPDQ dissolution from tire fragments and subsequent removal in response to added sorbents. This work was supported by infrastructure funded by the Canada Foundation for Innovation/British Columbia Knowledge Development Fund (32238 & 40274), operational support from the Natural Science and Engineering Research Council (NSERC) of Canada Discovery Grant Program funding RGPIN-2016-06454 (ETK), RGPIN-2021-02981 (CGG), and NSERC graduate (JM) and undergraduate (AJ) student scholarships. Post-print version https://viurrspace.ca/bitstream/handle/10613/26436/GillKroghESTL2021.pdf?sequence=3
Databáze: OpenAIRE