CYCLIN A IS REQUIRED IN S PHASE IN NORMAL EPITHELIAL CELL
| Autor: | Zindy, Frederique, Lamas, Eugenia, Chenivesse, Xavier, Sobczak, Joelle, Wang, Jian, Fesquet, Didier, Henglein, Berthold, Brechot, Christian |
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| Přispěvatelé: | St Jude Children's Research Hospital, CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Laboratoire d'Enzymologie des Acides Nucléiques, Université Pierre et Marie Curie - Paris 6 (UPMC), Centre de recherches de biochimie macromoléculaire (CRBM), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-IFR122-Centre National de la Recherche Scientifique (CNRS), Laboratoire de neurobiologie cellulaire et moléculaire (NBCM), Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 1992 |
| Předmět: | |
| Zdroj: | Biochemical and Biophysical Research Communications Biochemical and Biophysical Research Communications, 1992, 182 (3), pp.1144-1154 |
| ISSN: | 0006-291X 1090-2104 |
| Popis: | We have investigated cyclin A expression in a primaryculture of normal rat hepatocytes and during rat liverregeneration after partial hepatectomy. In both cases, cyclin AmRNA and protein accumulate as the cells enter S phase. Toinvestigate the potential implication of cyclin A accumulation atS phase, we microinjected anti-sense DNA constructs for cyclin A,resulting in effective inhibition of S phase entry. These effectswere specific for cyclin A since anti-sense cyclin B construct hadno similar effects. These results therefore, obtained in normalepithelial cells, indicate that cyclin A is involved in S phaseand thus should not be only considered as a mitotic cyclin. |
| Databáze: | OpenAIRE |
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