Research of epigenetic aspects of hematopoietic and spermatogenesis stem cells
| Autor: | Hybešová, Michaela |
|---|---|
| Přispěvatelé: | Pimková, Kristýna, Děd, Lukáš |
| Jazyk: | čeština |
| Rok vydání: | 2022 |
| Předmět: |
Remodelace chromatinu
progenitory proteomics RNA sequencing progenitors RNA sekvenování mass spectrometry kmenové buňky hmotnostní spektrometrie Chromatin remodeling mouse models spermatogonie spermatogonia ATP-dependent chromatin remodeling complexes hematopoéza spermatogenesis spermatogeneze Smarca5 ATP-dependentní komplexy remodelující chromatin proteomika hematopoiesis myší model stem cells Snf2h |
| Popis: | Stem cell differentiation is controlled by coordinated regulation of gene transcription. One of the regulatory factors is the loosening of chromatin and the accessibility of DNA to transcription factors. Chromatin remodeling is mediated by remodeling complexes. The ISWI chromatin remodeling ATPase Smarca5 (S5) is an important factor of remodeling complexes. It is a highly conserved chromatin-remodeling factor forming a catalytic subunit that can be found in several oligosubunit complexes. In these complexes, it actively regulates nucleosome structure and remodeling during DNA replication, repair and transcription. S5 has been identified as a key protein in embryonic development. Its deficiency leads to defects in hematopoiesis and male genital development. In the presented study, we focused on the role of S5 in hematopoiesis and spermatogenesis. Using a mouse model with transgenic expression of S5, co-immunoprecipitation and mass spectrometry, we identified S5 complexes in hematopoietic and testicular cells. We also studied the phenotypic consequences of S5 deficiency in mouse testes and found that it leads to impaired sperm development and male sterility. Using transcriptomic and proteomic analysis, we identified several molecular programs that could lead to reproductive disorders. Our work... |
| Databáze: | OpenAIRE |
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