| Popis: |
4 Abstract Nowadays, there is no standard method for determination of organic corrosion inhibitors in engine coolant. Due to that the aim of this bachelor thesis is to develop a rapid and effective method for their determination by UHPLC method in all commercially available engine coolants. The method was developed and optimized. The optimal separation conditions are: core-shell column Poroshell 120 SB-AQ, gradient elution programme of methanol and 0.05% (v/v) trifluoracetic acid solution mixture (concentration of methanol changes from 40 to 65 % (v/v)), dosed volume 1 µl, detection at wavelength 211 nm. All analyzes were performed at 25 řC. The analysis duration is 6.5 min. Calibration dependences were measured from 0.25 to 4.0 g l-1 for 2-ethylhexanoic, 3,5,5-trimethylhexanoic, nonanoic and decandioic acid, in range 0.025 - 0.400 g l-1 for imidazole, benzotriazole, methylbenzotriazole, benzothiazole-2-thiol and benzoic acid. The calibration curves are linear in whole range of detector for all chosen corrosion inhibitors. Key words engine coolants, organic acids, aryltrizoles, UHPLC, Core-Shell columns |
