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Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Kateřina Knoppová Supervisor: Doc. Ing. Barbora Szotáková, Ph.D. Supervisor specialist: RNDr. Věra Králová, Ph.D. Title of diploma thesis: Influence of albendazole on cell proliferation in vitro Albendazole (ABZ) is a benzimidazole anthelmintic widely used for the treatment of parasitic diseases in man and animals. The mechanism of action is based on inhibition of microtubule polymerization by binding of ABZ to the β-subunit of tubulin in parasites. As a result, it subsequently leads to the disruption of vital functions of the parasite and to its death. After elucidation of this mechanism, ABZ was tested as a potential anticancer agent. It has been shown that it is able to inhibit proliferation of some cancer cell lines. In this study, we examined the effect of ABZ on cell proliferation in vitro. For the purpose of our experiment we used cell lines derived from colorectal carcinoma - SW 480 and SW 620 together with a cell line NCM 460 isolated from normal colonic mucosa. The cells were treated with ABZ in the concentration range of 0.01 - 10 µM for 24, 48 and 72 hours. Cell viability was determined using WST-1 assay and Neutral Red Uptake Test (NRU). ABZ inhibited cell proliferation... |
