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University of Gothenburg; Charles University Sahlgrenska Academy; Faculty of Pharmacy in Hradec Králové Institute of Neuroscience and Physiology; Department of Pharmacology & Toxicology Student: Jan Rataj Supervisor: Michael Winder, Ph.D.; Özgü Aydogdu, M.D.; Prof. PharmDr. František Štaud, Ph.D. Title of diploma thesis: Functional receptor alterations in urinary bladder and urethra in an animal model of chronic prostatitis-chronic pelvic pain syndrome Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is a common urological disorder. Current available pharmacological treatment options are not effective enough, mostly due to the etiology of CP/CPPS not being fully understood. My thesis aimed to examine potential urethral changes, using immunohistochemistry methods and an organ bath setup, in a rat model of CP/CPPS. The thesis also examined changes in the expression of muscarinic receptor subtype 3 (M3), β3 adrenoceptors, and purinergic P2X1 and P2X3 receptors in the urinary bladder using immunohistochemistry. Specifically, it was examined how these differ when celecoxib, BAY 60-2770 and a combination of celecoxib and BAY 60-2770 had been administered during the development of prostate inflammation in the rat model of CP/CPPS. The data show an upregulation of M3 receptors in animals with... |
