Preparation and characterization of a bacterial protein YddV, a globin-coupled oxygen sensor diguanylate cyclase
| Autor: | Křížová, Věra |
|---|---|
| Přispěvatelé: | Martínková, Markéta, Man, Petr |
| Jazyk: | čeština |
| Rok vydání: | 2012 |
| Předmět: |
hem detekující proteiny
hem obsahující senzorové proteiny exprese proteinu heme sensor proteins isolation of plasmid DNA plyn detekující proteiny gas sensor proteins heme-containing sensor proteins a globin-coupled oxygen sensor with diguanylate cycklase activity (YddV) globin obsahující kyslíkový senzor s diguanylát cyklasovou aktivitou (YddV) izolace plasmidu expression of protein |
| Popis: | Heme-containing proteins are very important for proper function of various organisms, both prokaryotic and eukaryotic. The most well-known ones are myoglobin, hemoglobin and various peroxidases and cytochromes. The group of heme-containing proteins was recently extended by a new type of proteins, which are called heme-containing sensor proteins. These proteins consist of two domains, sensor domain, which is able to detect a signal (either a molecule of heme or a molecule of gas), and functional domain, which is often an enzyme or a transcriptional factor. Activity of the functional domain depends on the concentration of signal molecule, which binds to the sensor domain, which then causes change of its structure. Consequently, change of structure of the sensor domain also leads to change of structure of the functional domain. Heme-containing sensor proteins can be further divided into two smaller groups: heme sensor proteins and gas sensor proteins. Heme sensor proteins share a lot of features, for example binding of heme to a thiolate residue from cysteine, CP motif, etc. Gas sensor proteins detect a molecule of gas, especially oxygen, carbon monoxide and nitrogen monoxide. There have already been conducted various studies focusing on heme-containing sensor proteins, however, the mechanism of signal... |
| Databáze: | OpenAIRE |
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