| Popis: |
Turquoise killifish had to adapt to the inhospitable conditions in which they live, especially drying temporal water bodies, which means certain death. The life sprint of the representatives of this genus is at its peak within one-month post-hatching, when both sexes have fully developed gonads and can reproduce. This rate comes with a high cost, as the killifish gonads begin to show signs of tissue degradation and germ cell apoptosis as early as three months post-hatching. Germ cell loss increases with age. A description of the development and degradation of the gonads in males and their breeding was elaborated. For the evaluation of aging-specific changes, immunochemical methods were used, focusing on the binding of specific antibodies against target epitopes and their visualization using fluorescence microscopy. Procedures for histological specimens have also been described, both for classical light and fluorescence microscopy. |
