| Popis: |
Hepatitis B is an inflammatory viral disease of the liver. The etiological agent is a DNA virus from the Hepadnaviridae family. It is transmitted through the blood and secretions of an infected person. The greatest danger of the disease lies in the transition to chronicity. Chronic infection carries a high risk of developing liver cirrhosis and hepatocellular carcinoma. Hepatitis B is widespread throughout the world. The most reliable prevention is vaccination and avoidance of risky behavior and contact with persons with risky behavior, especially drug users and promiscuous persons. The aim of this bachelor thesis is to get acquainted with the disease of viral hepatitis B and to describe the current possibilities of diagnostics of hepatitis B markers using the COBAS e601 device from ROCHE by electrochemiluminescent immunoassay and quantification of viral DNA on the COBAS 4800 from ROCHE by real time PCR and evaluation of results using both methods. The set of examined samples comes from the laboratory of infectious serology and virology of the company VIDIA-DIAGNOSTIKA. The results show that HBsAg is the most investigated marker of hepatitis B. Positive findings make up only 0.67%. The ratio of HBsAg positive men and women in 2019 was almost balanced. Most HBsAg positive patients were aged 30-40 years. I also examined the prevalence of anti-HBc Ig total antibodies. The positivity of anti-HBc Ig total was confirmed in 4.27% of examinations. The ratio of anti-HBc Ig total positive men and women in 2019 was also almost balanced and the most positive were at the age of 30-40 years. |
