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Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Author: Bc. Barbora Červenková Supervisor: Mgr. Karolína Jankovičová, Ph.D. Title of diploma thesis: Possibilities of Cell Mediated Immunity Examination in Infertile Women Laboratory diagnostics in reproduction immunology has fast progressed during the last years. This work is focused on influence of various factors on cell mediated immunity, because immunological reasons of infertility and recurrent spontaneous abortions are one of the most often. It evaluates infulence of presence of antibodies, clinical data and partner's ejaculate quality on cells mediated immunity of examined women. It also compares two diagnostics procedures to evaluate cells immunity, the long-term migration-inhibition leukocytes test under agarosis with sperm's and trophoblast's antigens and the later NK cells activation test with sperm's and trophoblast's antigens analysed by flow cytometry. NK cells play an important role in etiology of recurrent spontaneous abortions, and that is why we observe their activation level. By the evaluated set of 305 infertile women,who were examined between years 2009 and 2011 in the Clinical Immunology and alergology department of the Hradec Králové's Faculty Hospital, has been... |
