| Autor: |
Miniati, A. Weng, Z. Zhang, B. Therianou, A. Vasiadi, M. and Nicolaidou, E. Stratigos, A. J. Antoniou, C. and Theoharides, T. C. |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
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| Popis: |
Vitiligo is a disorder of depigmentation, for which the pathogenesis is as yet unclear. Interleukin (IL)-8 (CXCL8) is a key inflammatory chemokine. We investigated the regulation of IL-8 production in human melanocytes, and the IL-8 serum levels and skin gene expression in patients with vitiligo and in controls. Cultured melanocytes were stimulated for 24h with tumour necrosis factor (TNF) 100ng/mL and IL-1 10ng/mL, with or without pretreatment with luteolin 50mol/L for 30min, and IL-8 release was measured by ELISA. Serum cytokines were measured by a microbead array. Skin biopsies were taken from healthy subjects (n=14) as well as from marginal lesional and nonlesional skin from patients with vitiligo (n=15). IL-8 gene expression was evaluated by quantitative real time PCR. Both TNF and IL-1 stimulated significant IL-8 release (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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